CAJ | 학술논문

通过对北京密云水库的野生鲤鱼的微耳石和星耳石中的蛋白质进行提取,分别得到了3种基质蛋白,根据其在提取过程中溶解性的不同,分为水可溶蛋白(WSP)、酸可溶蛋白(ASP)和酸不可溶蛋白(AIP).通过高效液相色谱、X射线衍射、红外光谱分析和氨基酸成分分析等方法分别对3种基质蛋白进行了表征,发现微耳石和星耳石中3种基质蛋白特征存在差异,并初步推断蛋白质特征的差别与碳酸钙晶体在微耳石和星耳石中的不同晶型有关.
통과대북경밀운수고적야생리어적미이석화성이석중적단백질진행제취,분별득도료3충기질단백,근거기재제취과정중용해성적불동,분위수가용단백(WSP)、산가용단백(ASP)화산불가용단백(AIP).통과고효액상색보、X사선연사、홍외광보분석화안기산성분분석등방법분별대3충기질단백진행료표정,발현미이석화성이석중3충기질단백특정존재차이,병초보추단단백질특정적차별여탄산개정체재미이석화성이석중적불동정형유관.
The proteins in lapillu and asteriscu of wild carp from the Miyun Reservoir in Beijing were extracted. According to the difference in solubility, the proteins can be classified into three groups, namely, water soluble protein( WSP), acid soluble protein( ASP) and acid insoluble protein(AIP). Using high performance liquid cliromatography(HPLC), X-ray diffraction(XRD) and Fourier transformation infrared spectra (FTIR), the authors characterized the sorted proteins from different otoliths. It is found that the three types of proteins in lapillus and asteriscus have different characteristics, and such differences may be responsible for different kinds of crystalline state in calcium carbonate.