CAJ | 학술논문

L-谷氨酸消旋化反应对于制备光学纯D-谷氨酸具有重要的意义.本文利用532 nm激光诱导荧光共聚焦检测式毛细管阵列电泳仪研究了L-谷氨酸基于席夫碱中间物的消旋化反应,采用羧基四甲基罗丹明琥珀酰亚胺酯(TAMRA)荧光探针标记消旋化产物后,以含有2 mmol/L 环糊精(β-CD)的100 mmol/L Tris-硼酸缓冲液(pH 10)为分离电解质进行毛细管阵列电泳分析,在该条件下TAMRA标记的谷氨酸对映体可以得到基线拆分.详细考察了醛的种类及用量、羧酸溶剂的种类、反应体系的含水量对消旋化的影响,结果表明L-谷氨酸在含20%(体积分数)水的乙酸溶剂中,以水杨醛作为催化剂(水杨醛与L-谷氨酸的物质的量比为0.2)的优化条件下可以快速地被消旋化.
L-곡안산소선화반응대우제비광학순D-곡안산구유중요적의의.본문이용532 nm격광유도형광공취초검측식모세관진렬전영의연구료L-곡안산기우석부감중간물적소선화반응,채용최기사갑기라단명호박선아알지(TAMRA)형광탐침표기소선화산물후,이함유2 mmol/L 배호정(β-CD)적100 mmol/L Tris-붕산완충액(pH 10)위분리전해질진행모세관진렬전영분석,재해조건하TAMRA표기적곡안산대영체가이득도기선탁분.상세고찰료철적충류급용량、최산용제적충류、반응체계적함수량대소선화적영향,결과표명L-곡안산재함20%(체적분수)수적을산용제중,이수양철작위최화제(수양철여L-곡안산적물질적량비위0.2)적우화조건하가이쾌속지피소선화.
L-Glutamic acid (Glu) racemization research is of great importance for the preparation of optical pure D-Glu.In the present work,we studied L-Glu racemization reaction through Schiff base intermediate by using capillary array electrophoresis (CAE) with 532 nm laser-induced confocal fluorescence detection.The racemization products were labeled by carboxytetramethylrhodamine succinimidyl ester (TAMRA) and analyzed in a 100 mmol/L Tris-borate buffer (pH 10) system containing 2 mmol/L β-CD by CAE.Under this condition,the TAMRA-Glu enantiomer can be completely separated.The influences of the type and dosage of the aldehyde,organic acids,as well as the water content in the reaction system on the racemization were examined in detail,and L-Glu was racemized quickly in the presence of 0.2 molar ratio of L-Glu to salicylaldehyde (as the catalyzer) in acetic acid containing 20%(v/v) water.