CAJ | 학술논문

目的树鼩可作为多种人类疾病研究的良好模型,但其免疫系统各类细胞表面标志、功能以及在疾病发生、发展过程中的作用和意义尚无系统研究.研究以获得树鼩调节性T细胞(Tr)相关分子CD127为目的,以分析其分子特征.方法提取树鼩外周血总RNA反转录,经巢式PCR扩增获得目的片段,进而以Discovery Studio等生物软件进行分析.结果扩增得到全长为1 392 bp的树鼩CD127编码序列,确定了现有数据库中缺失的一处未知片段.树鼩CD127与人、黑猩猩亲缘关系较近,其氨基酸序列具有较高保守性,蛋白质三维结构整体与人相似,但N糖基化位点数目以及电荷分布存在差异.结论所得序列能够编码具有正常功能的蛋白,全长序列为后续单克隆抗体制备奠定了基础,有助于鉴定树鼩Treg细胞以及相关疾病机理的研究.
목적 수구가작위다충인류질병연구적량호모형,단기면역계통각류세포표면표지、공능이급재질병발생、발전과정중적작용화의의상무계통연구.연구이획득수구조절성T세포(Tr)상관분자CD127위목적,이분석기분자특정.방법 제취수구외주혈총RNA반전록,경소식PCR확증획득목적편단,진이이Discovery Studio등생물연건진행분석.결과 확증득도전장위1 392 bp적수구CD127편마서렬,학정료현유수거고중결실적일처미지편단.수구CD127여인、흑성성친연관계교근,기안기산서렬구유교고보수성,단백질삼유결구정체여인상사,단N당기화위점수목이급전하분포존재차이.결론 소득서렬능구편마구유정상공능적단백,전장서렬위후속단극륭항체제비전정료기출,유조우감정수구Treg세포이급상관질병궤리적연구.
Objective Tree shrews is a good human diseases models.The cloning of its immune cellular markers is necessary.This study is to obtain Tree shrews CD127 for analyzing its molecular characteristics.Methods The CD127 gene was amplified from total RNA of tree shrews peripheral blood by RT-nested PCR and its biological characteristics were analyzed by biology software such as Discovery Studio.Results The fulllength CD127 cDNA encoding 1392bp of Tree shrews was cloned and one unknown fragment of tree shrews CD127 cDNA in the database was defined.Tree shrews CD127 amino acid sequence was conserved,and showed a close genetic relationship with Homo sapiens and Pan troglodytes.The structure of Tree shrews CD127 showed a similarity with human CD127,but some differences in the N-glyasylation sites number and the charge distribution have been detected.Conclusions The full-length sequence obtained in this study encodes Tree shrews CD127 which would function as its counterpart of the primate.It is useful for the preparation of antibody and studies of the role of Tree shrews Tr in human diseases.