中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
21期
4-6
,共3页
癌%肝细胞%甲胎蛋白类%肿瘤转移%聚合酶链反应
癌%肝細胞%甲胎蛋白類%腫瘤轉移%聚閤酶鏈反應
암%간세포%갑태단백류%종류전이%취합매련반응
Carcinoma%hepatocellular%Alpha-fetoprotein%Neoplasm metastasis%Polymerasechain reaction
目的 探讨肝癌患者外周血甲胎蛋白(AFP)mRNA的表达与术后生存和转移的关系.方法 收集2005年1月至2006年12月经过根治性手术切除的肝癌患者66例.提取外周血细胞总RNA,巢式PCR检测AFP mRNA的表达.术后随访60个月.结果 肝癌患者外周血AFPmRNA表达率为40.91％(27/66).肝癌患者外周血AFP mRNA的表达与有无微血管入侵和转移密切相关(P＜ 0.05或＜0.01),而与患者的性别、年龄、有无HBV感染、有无肝硬化、AFP浓度、肿瘤大小及数量、Edmondson分级无相关性(P＞0.05).AFP mRNA阳性肝癌患者术后1、2、≥3年的生存率分别是66.7％(18/27)、38.9％(7/18)、28.6％(2/7),AFP mRNA阴性肝癌患者分别为84.6％(33/39)、60.6％(20/33)、45.0％(9/20),两者比较差异有统计学意义(P＜0.01).结论 肝癌患者外周血AFPmRNA的检测可以提示肿瘤早期的微小转移,可以用来作为术后复发的预测指标.
目的 探討肝癌患者外週血甲胎蛋白(AFP)mRNA的錶達與術後生存和轉移的關繫.方法 收集2005年1月至2006年12月經過根治性手術切除的肝癌患者66例.提取外週血細胞總RNA,巢式PCR檢測AFP mRNA的錶達.術後隨訪60箇月.結果 肝癌患者外週血AFPmRNA錶達率為40.91％(27/66).肝癌患者外週血AFP mRNA的錶達與有無微血管入侵和轉移密切相關(P＜ 0.05或＜0.01),而與患者的性彆、年齡、有無HBV感染、有無肝硬化、AFP濃度、腫瘤大小及數量、Edmondson分級無相關性(P＞0.05).AFP mRNA暘性肝癌患者術後1、2、≥3年的生存率分彆是66.7％(18/27)、38.9％(7/18)、28.6％(2/7),AFP mRNA陰性肝癌患者分彆為84.6％(33/39)、60.6％(20/33)、45.0％(9/20),兩者比較差異有統計學意義(P＜0.01).結論 肝癌患者外週血AFPmRNA的檢測可以提示腫瘤早期的微小轉移,可以用來作為術後複髮的預測指標.
목적 탐토간암환자외주혈갑태단백(AFP)mRNA적표체여술후생존화전이적관계.방법 수집2005년1월지2006년12월경과근치성수술절제적간암환자66례.제취외주혈세포총RNA,소식PCR검측AFP mRNA적표체.술후수방60개월.결과 간암환자외주혈AFPmRNA표체솔위40.91％(27/66).간암환자외주혈AFP mRNA적표체여유무미혈관입침화전이밀절상관(P＜ 0.05혹＜0.01),이여환자적성별、년령、유무HBV감염、유무간경화、AFP농도、종류대소급수량、Edmondson분급무상관성(P＞0.05).AFP mRNA양성간암환자술후1、2、≥3년적생존솔분별시66.7％(18/27)、38.9％(7/18)、28.6％(2/7),AFP mRNA음성간암환자분별위84.6％(33/39)、60.6％(20/33)、45.0％(9/20),량자비교차이유통계학의의(P＜0.01).결론 간암환자외주혈AFPmRNA적검측가이제시종류조기적미소전이,가이용래작위술후복발적예측지표.
[Objective]To explore the correlation of the expression of alpha-fetoprotein(AFP)mRNA in the peripheral blood and postoperative survival and metastasis of patients with liver cancer.[Methods] A total of 66 patients with liver cancer who received radical resection surgery from January 2005to December 2006 was enrolled in this study.The cell total RNA was extracted from peripheral blood and the expression of AFP mRNA was detected by nested PCR.All the patients were followed up for 60 months after surgery.[Results]The expression rate of AFP mRNA in the peripheral blood was 40.91％(27/66).The expression of AFP mRNA in the peripheral blood in patients with liver cancer was significantly related to microvascular invasion and metastasis(P ＜ 0.05 or ＜ 0.01),but the expression had no relationship with sex,age,HBV infection,cirrhosis,AFP concentration,tumour size and number,and Edmondson grading(P＞0.05).The overall 1,2,and ＞ 3 years survival rates of patients with positive AFP mRNA after surgery were 66.7％(18/27),38.9％(7/18),28.6％(2/7),respectively.The overall 1,2,and ≥3 years survival rates of patients with negative AFP mRNA after surgery were 84.6％(33/39),60.6％(20/33),45.0％(9/20).There was statistical significance between the survival rates of AFP mRNA-negative patients and AFP mRNApositive patients(P ＜ 0.01).[Conclusions] The detection of AFP mRNA in the peripheral blood may provide clue for early microscopic metastasis.It can be a prediction index for postoperative recurrence.
