CAJ | 학술논문

目的建立一种新的免疫损伤性大鼠肝纤维化模型的制作方法.方法将Wistar大鼠,雌性,随机分为正常对照组(8只),人血白蛋白肝纤维化模型组(15只),兔肝匀浆肝纤维化模型组(15只).免疫攻击8周后处死大鼠,取血清检测肝功能生化指标、肝组织羟脯氨酸;评价病理学分级.结果与正常对照组比较,兔肝匀浆组大鼠肝脏重量显著增加,肝重/体重显著增加;白蛋白显著降低,而球蛋白显著增高;肝组织羟脯氨酸显著增高;人血白蛋白模型组大鼠肝纤维化程度大于3级占20.0%(2/10);兔肝肝纤维化程度匀浆大鼠模型组大于3级者,占73.3%(11/15),两组比较差异显著(X2=4.87,P=0.027).结论本研究建立了另一种兔肝匀浆诱导肝纤维化模型.该模型成模率高,纤维化明显而炎性介质反应相对较轻,是研究肝纤维化免疫损伤机制及药效学的又一重要工具.
목적 건립일충신적면역손상성대서간섬유화모형적제작방법.방법 장Wistar대서,자성,수궤분위정상대조조(8지),인혈백단백간섬유화모형조(15지),토간균장간섬유화모형조(15지).면역공격8주후처사대서,취혈청검측간공능생화지표、간조직간포안산;평개병이학분급.결과 여정상대조조비교,토간균장조대서간장중량현저증가,간중/체중현저증가;백단백현저강저,이구단백현저증고;간조직간포안산현저증고;인혈백단백모형조대서간섬유화정도대우3급점20.0%(2/10);토간간섬유화정도균장대서모형조대우3급자,점73.3%(11/15),량조비교차이현저(X2=4.87,P=0.027).결론 본연구건립료령일충토간균장유도간섬유화모형.해모형성모솔고,섬유화명현이염성개질반응상대교경,시연구간섬유화면역손상궤제급약효학적우일중요공구.
Objective To establish a method of a new type of liver fibrosis model in rats induced by repeated injection of rabbits' liver homogenate. Methods Female Wistar rats were randomly divided into a normal control group (8 rats), a human albumin induced liver fibrosis model group (15 rats) and a rabbits'liver homogenate induced liver fibrosis group (15 rats). The induction of liver fibrosis began with an immune sensitizing period (4 weeks) and was followed by an immune attacking period (8 weeks). After 8 weeks'attacking, all rats were sacrificed under anesthesia. Liver enzymes in serum and hydroxyproline in liver tissue were measured by standard methods and pathological scores were assessed by pathologists. Results The rats' liver weight, ratio of liver weight to body weight in the model group of liver homogenate were significantly increased compared with the normal control group. Serum globulin, tissue hydroxyproline were significantly increased, whereas serum albumin was significantly decreased in the homogenate group. There was only 20.0 percent of liver fibrosis score (2/10) exceeding a degree of 3 in the albumin group whereas 73.3 percent of that (11/15) were exceeding a degree of 3 in the homogenate group and the difference was significant (x2 = 4. 87,P = 0. 027). Conclusion In the study, we established a method of a new type of experimental liver fibrosis model in rats. The method has a significantly high success rate and this model can be used to study the mechanism of liver fibrosis and the efficacy of antifibrotic medicine.