CAJ | 학술논문

目的探讨采用胫骨近段髓腔注射不同浓度的ATCC25923金黄色葡萄球菌菌液诱导的骨髓炎模型与菌液浓度的关系.方法 36只健康新西兰大白兔随机分为4组,其中实验组A、B、C各10只,对照组6只.采用胫骨近段髓腔注射0.1 ml 5%鱼肝油酸钠和不同浓度的ATCC25923金黄色葡萄球菌菌液的方法诱导骨髓炎,其中A组注入0.1 ml菌液浓度为3×108 CFU/ml的ATCC25923金黄色葡萄球菌,B组注入0.1 ml菌液浓度为3×109 CFU/ml的ATCC25923金黄色葡萄球菌,C组注入0.3 ml菌液浓度为3×109 CFU/ml的ATCC25923金黄色葡萄球菌,对照组注入0.1 ml生理盐水.不用抗生素来预防菌血症,4周后采用大体观察,放射影像和组织病理学等方法对诱导的骨髓炎模型进行评价分级.结果所有大白兔都可以看到早期局部组织肿胀、病腿跛行,不同程度食欲减退,同时伴有体质量增长缓慢.造模后4周后,实验组动物出现不同程度的骨密度降低,骨小梁纤细,数目减少,骨小梁间隙加宽,骨皮质变薄,骨溶解,死骨形成,新骨形成;病理切片显示实验组动物出现不同程度的中性嗜酸性粒细胞浸润,骨髓腔内髓细胞不同程度坏死,间质出血;采用兔金黄色葡萄球菌骨髓炎模型严重程度分级大体标本观察和放射学检测标准对所诱导模型进行严重程度分级:A组属1～2级(其中属1级2只,属2级7只),B组属2～3级(其中属2级3只,属3级6只),C组属3～4级(其中属3级2只,属4级5只).结论采用胫骨近段髓腔注射细菌菌液的方法能够较好的诱导骨髓炎模型,模型的严重程度随注入的细菌量的增多而加重,兔金黄色葡萄球菌骨髓炎模型严重程度分级大体标本观察和放射学检测标准能够较好的反映骨髓炎的严重程度分级.
목적 탐토채용경골근단수강주사불동농도적ATCC25923금황색포도구균균액유도적골수염모형여균액농도적관계.방법 36지건강신서란대백토수궤분위4조,기중실험조A、B、C각10지,대조조6지.채용경골근단수강주사0.1 ml 5%어간유산납화불동농도적ATCC25923금황색포도구균균액적방법유도골수염,기중A조주입0.1 ml균액농도위3×108 CFU/ml적ATCC25923금황색포도구균,B조주입0.1 ml균액농도위3×109 CFU/ml적ATCC25923금황색포도구균,C조주입0.3 ml균액농도위3×109 CFU/ml적ATCC25923금황색포도구균,대조조주입0.1 ml생리염수.불용항생소래예방균혈증,4주후채용대체관찰,방사영상화조직병이학등방법대유도적골수염모형진행평개분급.결과 소유대백토도가이간도조기국부조직종창、병퇴파행,불동정도식욕감퇴,동시반유체질량증장완만.조모후4주후,실험조동물출현불동정도적골밀도강저,골소량섬세,수목감소,골소량간극가관,골피질변박,골용해,사골형성,신골형성;병리절편현시실험조동물출현불동정도적중성기산성립세포침윤,골수강내수세포불동정도배사,간질출혈;채용토금황색포도구균골수염모형엄중정도분급대체표본관찰화방사학검측표준대소유도모형진행엄중정도분급:A조속1～2급(기중속1급2지,속2급7지),B조속2～3급(기중속2급3지,속3급6지),C조속3～4급(기중속3급2지,속4급5지).결론 채용경골근단수강주사세균균액적방법능구교호적유도골수염모형,모형적엄중정도수주입적세균량적증다이가중,토금황색포도구균골수염모형엄중정도분급대체표본관찰화방사학검측표준능구교호적반영골수염적엄중정도분급.
Objective To analyze the relationship between the inoculation dose of the bacteria of Staphylococcus aureus ATCC25923 and the severity of experimental Staphylococcus aureus osteomyelitis in rabbits. Methods Thirty-six healthy New Zealand white rabbits were randomized into 4 groups: control group and experimental groups A, B, C. There were 10 rabbits in every experimental group and 6 rabbits in control group. Five percent sodium morrhuate (0. 1 ml) and serial dilutions of the bacteria of Staphylococcus aureus ATCC25923 [3 × 108 to 3 × 109 colony-forming units ( CFU)/ml]suspended in saline or saline alone were inoculated into the proximal metaphysis of the tibia. No antibiotics were used to prevent fatal sepsis. The severity of experimental Staphylococcus aureus osteomyelitis in rabbits was evaluated by clinical, radiologic,bacteriologic and histologic parameters at the 4th week after infection. Results There was clinical progression of the disease observed by draining wounds, a postoperative limp that subsided in all rabbits, and varied periods of anorexia despite an average increase in body weight. There were varying degrees of BMD decrease, decreased bone trabeculae and bone trabecular space widening in experimental groups at the 4th week after infection.Slices indicated that there were varying degrees of neutrophil infiltration, necrosis of marrow cells and interstitial hemorrhage. Gross pathologic and radiographic criteria were used to grade the severity of experimental staphylococcus aureus osteomyelitis in rabbits. The grading of experimental groups A, B, and C was respectively stage 1 to 2 osteomyelitis, stage 2 to 3 osteomyelitis, stage 3 to 4 osteomyelitis. Conclusion The bacteria of Staphylococcus aureus ATCC25923 suspended in saline was inoculated into the proximal metaphysis of the tibia and can induce osteomyelitis. The severity of experimental Staphylococcus aureus osteomyelitis in rabbits was aggravated with the increase of the bacteria amount inoculated. The gross pathologic and radiographic criteria can preferably reflect the grading of severity of experimental Staphylococcus aureusosteomyelitis in rabbits.