CAJ | 학술논문

目的探讨应用荧光原位杂交(FISH)技术检测尿液脱落上皮细胞3、7、9、17号染色体畸变对于膀胱癌诊断的价值.方法分别对30例膀胱癌患者和20例非肿瘤血尿患者应用FISH试剂盒检测膀胱癌,使用荧光标记的DNA探针与3、7和17号染色体的着丝粒区以及9p21位点相结合,检测尿路上皮细胞基因的改变.在组织学诊断的基础上评估FISH检测的价值.结果 30例膀胱癌患者FISH检测的特异性和敏感性分别为100%和97%.结论 FISH是一种无创的膀胱癌诊断方法,具有快速、准确、灵敏度高的优点,对膀胱癌的诊断具有重要的临床应用价值.
목적 탐토응용형광원위잡교(FISH)기술검측뇨액탈락상피세포3、7、9、17호염색체기변대우방광암진단적개치.방법 분별대30례방광암환자화20례비종류혈뇨환자응용FISH시제합검측방광암,사용형광표기적DNA탐침여3、7화17호염색체적착사립구이급9p21위점상결합,검측뇨로상피세포기인적개변.재조직학진단적기출상평고FISH검측적개치.결과 30례방광암환자FISH검측적특이성화민감성분별위100%화97%.결론 FISH시일충무창적방광암진단방법,구유쾌속、준학、령민도고적우점,대방광암적진단구유중요적림상응용개치.
Objective To evaluate the diagnostic value of chromosome aberration of urine exfoliated cells by fluorescence in situ hybridization ( FISH ) for patients with bladder carcinoma. Methods Using centromeric probes of chromosomes 3, 7, 17 and region probe of 9p21, we performed FISH on the exfoliative cells in the urinary samples from 30 patients with pathologically confirmed bladder carcinoma and 20 hematuria patients with benign bladder disease controls. Then we noted and analyzed aberrations in the chromosomes. Results The sensitivity and specificity of FISH on detecting bladder cancer were 97% and 100%, respectively. Conclusions It is noninvasive and effective to diagnose bladder cancer with the techique of FISH. It is a rapid, accurate and very sensitive method and can be used in clinical diagnosis.