CAJ | 학술논문

目的研究缺氧程度与瘢痕疙瘩中缺氧诱导因子-1α(hypoxia inducible factor,HIF-1α)基因表达的量化关系和关联性,进一步探讨缺氧通过HIF-1α途径促进异常瘢痕形成的机制.方法设立不同O2浓度组,在常氧及不同程度缺氧条件下培养瘢痕疙瘩成纤维细胞,应用Western印迹技术检测比较各组间HIF-1α基因蛋白的表达,进行数据处理和统计学分析.结果瘢痕疙瘩成纤维细胞在常氧(20%)、不同缺氧浓度(10 %、5%、1%)下,HIF-1α蛋白表达相对含量(HIF-1α/β肌动蛋白)分别为0.007±0.006、0.133±0.006、0.537±0.015和0.903±0.021,表明缺氧系统中瘢痕疙瘩成纤维细胞HIF-1α蛋白表达明显上调.结论缺氧条件促进了瘢痕疙瘩中HIF-1α蛋白的表达,而且表达的量与缺氧程度呈正相关.缺氧通过HIF-1α基因途径与异常瘢痕的形成密切相关.
목적 연구결양정도여반흔흘탑중결양유도인자-1α(hypoxia inducible factor,HIF-1α)기인표체적양화관계화관련성,진일보탐토결양통과HIF-1α도경촉진이상반흔형성적궤제.방법 설립불동O2농도조,재상양급불동정도결양조건하배양반흔흘탑성섬유세포,응용Western인적기술검측비교각조간HIF-1α기인단백적표체,진행수거처리화통계학분석.결과 반흔흘탑성섬유세포재상양(20%)、불동결양농도(10 %、5%、1%)하,HIF-1α단백표체상대함량(HIF-1α/β기동단백)분별위0.007±0.006、0.133±0.006、0.537±0.015화0.903±0.021,표명결양계통중반흔흘탑성섬유세포HIF-1α단백표체명현상조.결론 결양조건촉진료반흔흘탑중HIF-1α단백적표체,이차표체적량여결양정도정정상관.결양통과HIF-1α기인도경여이상반흔적형성밀절상관.
Objective To investigate the correlation of HIF-1α and hypoxia in keloids fibroblasts, and to investigate the mechanism that hypoxia promotes abnormal scarring by HIF-1α pathway. Methods Keloid fibroblasts cultured in vitro were placed in an incubator with different O2 concentrations. After 24 h, the keloid fibroblasts were collected for further study. Western blotting was performed to detect the expression of HIF-1α in the keloid fibroblasts. Results Relative amounts of HIF-1α in keloid fibroblasts cultured under O2 concentrations at 20 %, 10 %, 5 % and 1 % were 0. 007 ±0. 006, 0. 133 ±0. 006, 0. 537±0. 015 and 0. 903±0. 021, respectively. It indicated that hypoxia could increase the expression of HIF-lα in keloid fibroblasts. Conclusions Hypoxia can induce the expression of HIF-1α in fibroblasts of keloids. Moreover, there still is a positive relation between hypoxia and the expression of HIF-1α. Therefore, a close relationship exists between abnormal scarring and HIF-1α pathway by hypoxia.