中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
6期
644-648
,共5页
酵母菌%RNA,核糖体,18S%序列分析,RNA
酵母菌%RNA,覈糖體,18S%序列分析,RNA
효모균%RNA,핵당체,18S%서렬분석,RNA
Yeasts%RNA,ribosomal,18S%Sequence analysis,RNA
目的 应用18S rRNA基因序列分析技术对临床分离的常见酵母样真菌进行种的分类鉴定,且与传统方法比较,分析基因序列分析法鉴定临床常见酵母样真菌的可行性.方法 收集北京同仁医院微生物室菌库酵母样真菌115株,提取的DNA用18S rRNA通用引物进行PCR扩增,扩增产物直接测序,测序结果提交GenBank通过核酸序列比对对微生物种属进行鉴定,同时进行真菌显色培养基鉴定、Vitek 32 YBC鉴定,比较3种不同方法鉴定酵母样真菌的种鉴定准确率,阐明应用基因序列分析法鉴定临床酵母样真菌的可行性.结果 18S rRNA基因序列分析法与表型鉴定法相比较,有13株酵母样真菌鉴定结果存在差异:显色培养基仅鉴定出102株,与基因序列分析鉴定的符合率为89.2%(91/102),Vitek 32 YBC鉴定结果与基因序列分析鉴定的符合率为91.3%(105/115);显色培养基、Vitek 32 YBC和18S rRNA基因序列分析鉴定到种的比例分别为88.7%(102/115)、100%(115/115)、100%(115/115).结论 18S rRNA基因序列分析法呵对常见酵母样真菌进行准确分类.
目的 應用18S rRNA基因序列分析技術對臨床分離的常見酵母樣真菌進行種的分類鑒定,且與傳統方法比較,分析基因序列分析法鑒定臨床常見酵母樣真菌的可行性.方法 收集北京同仁醫院微生物室菌庫酵母樣真菌115株,提取的DNA用18S rRNA通用引物進行PCR擴增,擴增產物直接測序,測序結果提交GenBank通過覈痠序列比對對微生物種屬進行鑒定,同時進行真菌顯色培養基鑒定、Vitek 32 YBC鑒定,比較3種不同方法鑒定酵母樣真菌的種鑒定準確率,闡明應用基因序列分析法鑒定臨床酵母樣真菌的可行性.結果 18S rRNA基因序列分析法與錶型鑒定法相比較,有13株酵母樣真菌鑒定結果存在差異:顯色培養基僅鑒定齣102株,與基因序列分析鑒定的符閤率為89.2%(91/102),Vitek 32 YBC鑒定結果與基因序列分析鑒定的符閤率為91.3%(105/115);顯色培養基、Vitek 32 YBC和18S rRNA基因序列分析鑒定到種的比例分彆為88.7%(102/115)、100%(115/115)、100%(115/115).結論 18S rRNA基因序列分析法呵對常見酵母樣真菌進行準確分類.
목적 응용18S rRNA기인서렬분석기술대림상분리적상견효모양진균진행충적분류감정,차여전통방법비교,분석기인서렬분석법감정림상상견효모양진균적가행성.방법 수집북경동인의원미생물실균고효모양진균115주,제취적DNA용18S rRNA통용인물진행PCR확증,확증산물직접측서,측서결과제교GenBank통과핵산서렬비대대미생물충속진행감정,동시진행진균현색배양기감정、Vitek 32 YBC감정,비교3충불동방법감정효모양진균적충감정준학솔,천명응용기인서렬분석법감정림상효모양진균적가행성.결과 18S rRNA기인서렬분석법여표형감정법상비교,유13주효모양진균감정결과존재차이:현색배양기부감정출102주,여기인서렬분석감정적부합솔위89.2%(91/102),Vitek 32 YBC감정결과여기인서렬분석감정적부합솔위91.3%(105/115);현색배양기、Vitek 32 YBC화18S rRNA기인서렬분석감정도충적비례분별위88.7%(102/115)、100%(115/115)、100%(115/115).결론 18S rRNA기인서렬분석법가대상견효모양진균진행준학분류.
Objective To compare sequence analysis of the yeast-like fungal isolates with traditional methods and analyze the feasibility of identification of common yeast-like fungal by sequence analysis of gene. Methods 115 yeast-like fungal isolates were collected in the clinical laboratory of Beijing Tongren Hospital. DNA of yeast-like fungal was extracted and then amplified with universal primers of part of 18S rRNA genes followed by sequencing directly. The sequences obtained were submitted to the GenBank (NCBI) to identify the fungi. At the same time, the CHROMagar Candida and Vitek 32 YBC were used to identify the fungi. The identification accuracy with three methods was compared to explore the feasibility of the identification of sequence analysis. Results 18S rRNA gene sequence analysis was compared with traditional method. There were some differences in the identification results of 13 strains. The coincidence rate between CHROMagar Candida and sequence analysis was 89. 2% (91/102) and the coincidence rate between Vitek 32 YBC and sequence analysis was 91.3% (105/115). The positivity rate of species-level identification by CHROMagar Candida , Vitek 32 YBC and the 18S rRNA gene sequence analysis were 88. 7 % ( 102/115 ), 100% ( 115/115 ), 100% ( 115/115 ). Conclusion Identification of medically important yeast-like fungal by sequence analysis of the 18S rRNA gene is reliability.
