CAJ | 학술논문

目的探讨结肠癌肿瘤超声抗原致敏的树突状细胞(DC)疫苗体外对结肠癌细胞的杀伤作用.方法分离HLA-A*0201型健康供者的外周血单个核细胞(PBMC),重组人细胞因子rhGM-CSF、rh IL-4、rh TNF-α体外诱导DC成熟,负载SW480超声抗原后体外刺激同一供者的细胞毒性T淋巴细胞(CTL)前体,MTT法检测CTL杀伤SW480(HLA-A*0201阳性)、K562(HLA-A*0201阴性)的细胞毒实验.结果在效靶比20∶1、10∶1、5∶1、2.5∶1作用时,CTL对SW480的杀伤率分别为51.12%、46.67%、52.60%、40.00%;对K562的杀伤率分别为29.26%、31.98%、39.46%、31.30%,证明在各种效靶比作用时,CTL对SW480的作用均强于K562(P＜0.05).结论肿瘤超声抗原致敏的DC瘤苗能激发具有特异杀伤功能的CTL,并且体外杀伤靶细胞具有MHC限制性.
목적 탐토결장암종류초성항원치민적수돌상세포(DC)역묘체외대결장암세포적살상작용.방법분리HLA-A*0201형건강공자적외주혈단개핵세포(PBMC),중조인세포인자rhGM-CSF、rh IL-4、rh TNF-α체외유도DC성숙,부재SW480초성항원후체외자격동일공자적세포독성T림파세포(CTL)전체,MTT법검측CTL살상SW480(HLA-A*0201양성)、K562(HLA-A*0201음성)적세포독실험.결과재효파비20∶1、10∶1、5∶1、2.5∶1작용시,CTL대SW480적살상솔분별위51.12%、46.67%、52.60%、40.00%;대K562적살상솔분별위29.26%、31.98%、39.46%、31.30%,증명재각충효파비작용시,CTL대SW480적작용균강우K562(P＜0.05).결론종류초성항원치민적DC류묘능격발구유특이살상공능적CTL,병차체외살상파세포구유MHC한제성.
Objective To explore the cytotoxicity of the cytotoxic T lymphocyte (CTL) induced by SW480 sonicate sensitized dendritic cells (DC) on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A*0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor's primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 (HLA-A*0201 positive) and K562 (HLA-A*0201 negative) was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 (P ＜0.05). Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.