CAJ | 학술논문

探讨玄参提取物(SnPs)对局灶性缺血后脑组织的保护作用及对脑血流量的影响.方法:雄性SD大鼠(242±s 7)g 40只随机分5组(均n=8):假手术组、模型对照组、3个不同剂量SnPs治疗组(1,5,10 mg·kg-1尾静脉注射).大脑中动脉线栓法建立局灶性脑缺血模型(MCAO),缺血24h后,用神经功能评分和梗死体积改变来评价SnPs对脑缺血的治疗作用.另将MCAO模型缺血SD大鼠分成4组(模型对照组n=8,3个不同剂量1,5,10 mg·kg-1SnPs治疗组均n=6)用于测脑血流量,用激光多普勒仪分别在6个时间点(缺血前,缺血即刻,缺血后30 min和2 h,以及缺血2 h再灌30min,2 h)记录双侧的皮层血流量.结果:脑缺血24h后,各SnPs治疗组与模型对照组相比,脑梗死体积明显减少(P＜0.05),神经功能均明显改善(P＜0.01);但在此剂量范围内无明显的剂量依赖性.各SnPs治疗组与模型组相比,缺血2 h后皮层CBF明显改善(P＜0.05),其中5 mg·kg-1剂量组对于缺血各时间点的血流改善均有显著作用(P＜0.05).结论:SnPs对于脑缺血损伤具有保护作用,此作用可能与提高脑血流量有关.
탐토현삼제취물(SnPs)대국조성결혈후뇌조직적보호작용급대뇌혈류량적영향.방법:웅성SD대서(242±s 7)g 40지수궤분5조(균n=8):가수술조、모형대조조、3개불동제량SnPs치료조(1,5,10 mg·kg-1미정맥주사).대뇌중동맥선전법건립국조성뇌결혈모형(MCAO),결혈24h후,용신경공능평분화경사체적개변래평개SnPs대뇌결혈적치료작용.령장MCAO모형결혈SD대서분성4조(모형대조조n=8,3개불동제량1,5,10 mg·kg-1SnPs치료조균n=6)용우측뇌혈류량,용격광다보륵의분별재6개시간점(결혈전,결혈즉각,결혈후30 min화2 h,이급결혈2 h재관30min,2 h)기록쌍측적피층혈류량.결과:뇌결혈24h후,각SnPs치료조여모형대조조상비,뇌경사체적명현감소(P＜0.05),신경공능균명현개선(P＜0.01);단재차제량범위내무명현적제량의뢰성.각SnPs치료조여모형조상비,결혈2 h후피층CBF명현개선(P＜0.05),기중5 mg·kg-1제량조대우결혈각시간점적혈류개선균유현저작용(P＜0.05).결론:SnPs대우뇌결혈손상구유보호작용,차작용가능여제고뇌혈류량유관.
AIM: To investigate the protectiveeffect of Scrophularia ningpoensis extracts (SnPs)on focal cerebral ischemic and the influence on cerebral blood flow (CBF). METHODS: MaleSprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO). Forty rats were randomly divided into five groups (n = 8), sham operation group, model group and SnPs-treatment groupsscores were determined 24 h after MCAO (grading of0 to 4). Ischemic lesion volume was measured by 2,3,5 -triphenyltetrazolium chloride (TTC) staining.CBF was monitored at six time points: beforeMCAO; instant, 0.5 h, 2 h after MCAO and 0.5 h,2 h after reperfusion performed (2 h after MCAO) incontrol group ( received saline). RESULTS: TheSnPs-treated groups showed significant improvementin neurological deficits compared with control group(P＜0.01). Lesion volume was (15 ± s 4) % , (14±5) %, (15±3) % and (22±4) % in three treated groups and control groups, respectively. The lesion volume was significantly decreased 24 h afterMCAO in SnPs groups compared with the controlgroup (P ＜ 0.05). In comparison to CBF decrease incontrol group, CBF was significantly improved in theIn other two treated groups, CBF was obviously improved only at the time point of 2 h after MCAO (P＜ 0.05). CONCLUSION: SnPs has protectiveeffect on cerebral ischemia injury and this effect maybe associated with enhancement of CBF recovery.