CAJ | 학술논문

将海藻酸钠-壳聚糖固定化磷脂酶用于水化大豆油脱胶,通过单因素实验和旋转正交实验,确定最佳脱胶条件为:固定化酶添加量0.012%,起始pH 5.8,反应温度58 ℃,反应时间4 h.在最优条件下进行脱胶实验,测定固定化酶法脱胶油中的磷含量为3.85 mg/kg,脱胶效果较好.同时,固定化磷脂酶热稳定性提高,对反应环境的耐受力明显增强,使脱胶反应条件更易控制,是一种非常有效的脱胶方法.
장해조산납-각취당고정화린지매용우수화대두유탈효,통과단인소실험화선전정교실험,학정최가탈효조건위:고정화매첨가량0.012%,기시pH 5.8,반응온도58 ℃,반응시간4 h.재최우조건하진행탈효실험,측정고정화매법탈효유중적린함량위3.85 mg/kg,탈효효과교호.동시,고정화린지매열은정성제고,대반응배경적내수력명현증강,사탈효반응조건경역공제,시일충비상유효적탈효방법.
The sodium alginate-chitosan immobilized phospholipase(PLA) was used for degumming of soybean oil. Through single factor test and orthogonal test, the optimal reaction conditions were determined as follows:immobilized enzyme dosage 0.012%, initial pH 5.8, reaction temperature 58 ℃, reaction time 4 h. Under the optimal conditions, the phosphorus content reduced to 3.85 mg/kg in degummed soybean oil. In addition, the thermal stabilities and the tolerance to the reaction conditions of immobilized PLA were enhanced, and the degumming conditions in practical production were easy to control.