CAJ | 학술논문

利用PCR技术克隆西花蓟马(Frankliniella occidentalis)的rDNA ITS2和COⅠ基因5'末端的部分序列,结合GenBank中相应序列对两者的突变类型、核苷酸多态性、单倍型多态性、种内平均遗传距离以及系统进化等进行比较分析.结果表明,COⅠ基因较rDNA ITS2更适合于F. occidentalis的种内遗传分析;F. occidentalis可能是由两个(或多个)株系或隐存种组成的复合体;入侵我国的F. occidentalis以温室系(greenhouse strain)为主体,也存在一定数量的羽扇豆系(lupin strain).据此,推测F. occidentalis最先是被传播至云南,再从云南扩散至北京、哈尔滨等地.本研究结果对了解我国F. occidentalis的遗传多样性、株系组成以及入侵机制具有一定意义.
이용PCR기술극륭서화계마(Frankliniella occidentalis)적rDNA ITS2화COⅠ기인5'말단적부분서렬,결합GenBank중상응서렬대량자적돌변류형、핵감산다태성、단배형다태성、충내평균유전거리이급계통진화등진행비교분석.결과표명,COⅠ기인교rDNA ITS2경괄합우F. occidentalis적충내유전분석;F. occidentalis가능시유량개(혹다개)주계혹은존충조성적복합체;입침아국적F. occidentalis이온실계(greenhouse strain)위주체,야존재일정수량적우선두계(lupin strain).거차,추측F. occidentalis최선시피전파지운남,재종운남확산지북경、합이빈등지.본연구결과대료해아국F. occidentalis적유전다양성、주계조성이급입침궤제구유일정의의.
Using PCR technique, both rDNA ITS2 and 5' terminal sequence of COⅠgenes of Frankliniella occidentalis were cloned and sequenced. Based on nucleotide sequences obtained in the present and from GenBank, the mutation type, nucleotide diversity, haplotype diversity and average intra-species genetic distance of rDNA ITS2 and COⅠ were analyzed and compared. Results show that COⅠ was a better candidate gene for detecting intra-specific polymorphisms, and to characterize genetic diversity;F. occidentalis may consist of two (or more) strains or cryptic (sub) species, and F. occidentalis population in China consisted of both greenhouse strain and lupin strain, with greenhouse strain as majority. Based on the knowledge thus far, we presumed that the first invaded area of F. occidentalis was Yunnan Province, and from there this exotic invasive insect dispersed to other parts of China, such as Beijing and Harbin. These results provide a basis for understanding the genetic diversity, strain composition and invasion mechanism of F. occidentalis in China.