CAJ | 학술논문

采用气相色谱-质谱(GC-MS)分析了大鼠尿液中的异丙嗪及其代谢产物.尿液经β-葡萄糖醛酸酶酶解,Oasis~((R)) HLB 3 mL固相萃取柱提取后,以Restek Rtx-5MS(30 m×0.25 mm×0.25 μm)石英毛细管色谱柱为分析柱,经GC-MS分离检测.根据质谱图对代谢物的结构进行鉴定.结果表明:在大鼠尿液中检出了吩噻嗪、去甲基异丙嗪、异丙嗪亚砜、2-羟基去甲基异丙嗪、二羟基异丙嗪、顺-N-(1-丙烯基)吩噻嗪、反-N-(1-丙烯基)吩噻嗪、顺-N-(1-丙烯基)吩噻嗪砜、反-N-(1-丙烯基)吩噻嗪砜、顺-N-(1-丙烯基)吩噻嗪亚砜及反-N-(1-丙烯基)吩噻嗪亚砜11种代谢物.对异丙嗪在大鼠体内的氧化、还原等主要代谢途径进行了讨论.
채용기상색보-질보(GC-MS)분석료대서뇨액중적이병진급기대사산물.뇨액경β-포도당철산매매해,Oasis~((R)) HLB 3 mL고상췌취주제취후,이Restek Rtx-5MS(30 m×0.25 mm×0.25 μm)석영모세관색보주위분석주,경GC-MS분리검측.근거질보도대대사물적결구진행감정.결과표명:재대서뇨액중검출료분새진、거갑기이병진、이병진아풍、2-간기거갑기이병진、이간기이병진、순-N-(1-병희기)분새진、반-N-(1-병희기)분새진、순-N-(1-병희기)분새진풍、반-N-(1-병희기)분새진풍、순-N-(1-병희기)분새진아풍급반-N-(1-병희기)분새진아풍11충대사물.대이병진재대서체내적양화、환원등주요대사도경진행료토론.
A gas chromatography and mass spectrometric(GC-MS) method was developed for the analysis of promethazine(PMZ) and its metabolites in wistar rat with the electron ionization(EI) and the positive chemical ionization(PCI) mode.The sample was hydrolyzed with β-glucuronidase,and extracted by a Oasis~((R)) HLB 3 mL solid phase extraction(SPE).The separation of target compounds was performed on a Restek Rtx-5 MS(30 m×0.25 mm×0.25 μm) capillary column.The molecular structures of the metabolites were identified based on their MS spectra.The result indicated that PMZ and its eleven metabolites,including phenothiazine,desmethylpromethazine,promethazine sulphoxide,2-hydroxy-desmethylpromethazine,dihydroxypromethazine,cis-N-(1-propenyl) phenothiazine,trans-N-(1-propenyl) phenothiazine,cis-N-(1-propenyl) phenothiazine-5-oxide,trans-N-(1-propenyl) phenothiazine-5-oxide,cis-N-(1-propenyl) phenothiazine-5,5-dioxide,trans-N-(1-propenyl) phenothiazine-5,5-dioxide were found in rat urine.The main metabolic process of PMZ in rat bio-body was also discussed.