CAJ | 학술논문

用实时荧光定量PCR方法研究了蓝塘猪和长白猪1、27、90、150和180日龄阶段脂肪组织中leptin基因mRNA和下丘脑中leptin受体基因mRNA的变化,并对2个品种不同生长阶段血液中leptin含量的变化规律进行了探讨.结果表明:(1)蓝塘猪180日龄时皮下脂肪中leptin基因表达量显著高于其它各阶段,而与长白猪相比,蓝塘猪除1日龄低于长白猪外,其它各个阶段均高于长白猪,180日龄两者差异极显著;长白猪各阶段间差异不显著;(2)蓝塘猪180日龄时下丘脑中leptin受体基因的表达量显著高于其它各阶段,长白猪180日龄时最高,且显著高于1日龄和27日龄,蓝塘猪与长白猪1日龄时差异显著;(3)蓝塘猪血清中leptin浓度各阶段均高于长白猪,两品种均是150日龄时最高,蓝塘猪150日龄显著高于27日龄和180日龄,长白猪各阶段差异不显著.
용실시형광정량PCR방법연구료람당저화장백저1、27、90、150화180일령계단지방조직중leptin기인mRNA화하구뇌중leptin수체기인mRNA적변화,병대2개품충불동생장계단혈액중leptin함량적변화규률진행료탐토.결과표명:(1)람당저180일령시피하지방중leptin기인표체량현저고우기타각계단,이여장백저상비,람당저제1일령저우장백저외,기타각개계단균고우장백저,180일령량자차이겁현저;장백저각계단간차이불현저;(2)람당저180일령시하구뇌중leptin수체기인적표체량현저고우기타각계단,장백저180일령시최고,차현저고우1일령화27일령,람당저여장백저1일령시차이현저;(3)람당저혈청중leptin농도각계단균고우장백저,량품충균시150일령시최고,람당저150일령현저고우27일령화180일령,장백저각계단차이불현저.
Real time RT-PCR was applied to study the developmental patterns of leptin and leptin receptor gene mRNA expression at the ages of 1,27,90,150 and 180 days after born (DAB) in subcutaneous fat and hypothalamus of Lantang and Landrace pigs.The results showed that:(1) in subcutaneous fat,Lantang had the highest leptin mRNA level at 180 DAB.Leptin mRNA at all ages in lantang was higher than that in landrace,except at 1 DAB.Leptin mRNA was significantly higher in Lantang pig than that in landrace pig at 180 DAB.Leptin mRNA had no significant difference among landrace pigs at all ages.(2) In hypothalamus,leptin receptor mRNA was highest at 180 DAB for both the two breeds,and it had significantly higher than that at other ages in lantang,and at 1 and 27 d in landrace.Two breeds at 1 d had apparent differed.(3) Lantang pigs at all ages had higher serum leptin level than landrace.Two breeds at 150 d were the highest compared with other ages.Lantang pigs at 150 day had significantly higher than at ages of 27 and 180 day.No significant changes were seen in all ages in landrace.