四川大学学报(医学版)
四川大學學報(醫學版)
사천대학학보(의학판)
JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES
2010年
1期
73-76
,共4页
邱永洁%张浩%孙丁%邱云良%王津涛
邱永潔%張浩%孫丁%邱雲良%王津濤
구영길%장호%손정%구운량%왕진도
二巯基敌枯双%FRTL-5细胞%甲状腺球蛋白%甲状腺过氧化物酶%超微结构
二巰基敵枯雙%FRTL-5細胞%甲狀腺毬蛋白%甲狀腺過氧化物酶%超微結構
이구기활고쌍%FRTL-5세포%갑상선구단백%갑상선과양화물매%초미결구
N' N-methylene-bis%FRTL-5 cell%Thyroglobulin%Thyroid peroxidases%Ultrastructure
目的 观察农药二巯基敌枯双对FRTL-5细胞株合成甲状腺球蛋白的影响,并探讨其作用机制以及FRTL-5细胞株用于筛选环境甲状腺素干扰物的可能性.方法 FRTL-5细胞分别经0.1、1.0和10.0 μg/mL二巯基敌枯双染毒处理48 h后,用酶组织化学法测定培养液中甲状腺球蛋白的浓度;用活细胞爬片的酶细胞化学染色观察细胞内甲状腺过氧化物酶的表达强度,并用透射电镜观察最高浓度处理组细胞的超微结构.结果 随二巯基敌枯双浓度的增大,FRTL-5细胞培养液中甲状腺球蛋白的浓度逐渐降低,其中0.1、1.0 μg/mL浓度组与溶剂对照组相比差异有统计学意义(P＜0.05),10.0 μg/mL浓度组培养液中未能检出甲状腺球蛋白;酶细胞化学染色未见染毒组细胞甲状腺过氧化物酶活性改变,图像分析结果 显示其阳性细胞的积分光密度(IOD)值与对照组相比,差异无统计学意义(P＞0.05);细胞超微结构观察发现染毒组细胞出现粗面内质网扩张.结论 二巯基敌枯双可能是通过损伤甲状腺滤泡细胞的粗面内质网,从而影响甲状腺球蛋白的合成与分泌;FRTL-5细胞培养液中甲状腺球蛋白的浓度可以作为体外筛选环境甲状腺素干扰物的敏感指标之一.
目的 觀察農藥二巰基敵枯雙對FRTL-5細胞株閤成甲狀腺毬蛋白的影響,併探討其作用機製以及FRTL-5細胞株用于篩選環境甲狀腺素榦擾物的可能性.方法 FRTL-5細胞分彆經0.1、1.0和10.0 μg/mL二巰基敵枯雙染毒處理48 h後,用酶組織化學法測定培養液中甲狀腺毬蛋白的濃度;用活細胞爬片的酶細胞化學染色觀察細胞內甲狀腺過氧化物酶的錶達彊度,併用透射電鏡觀察最高濃度處理組細胞的超微結構.結果 隨二巰基敵枯雙濃度的增大,FRTL-5細胞培養液中甲狀腺毬蛋白的濃度逐漸降低,其中0.1、1.0 μg/mL濃度組與溶劑對照組相比差異有統計學意義(P＜0.05),10.0 μg/mL濃度組培養液中未能檢齣甲狀腺毬蛋白;酶細胞化學染色未見染毒組細胞甲狀腺過氧化物酶活性改變,圖像分析結果 顯示其暘性細胞的積分光密度(IOD)值與對照組相比,差異無統計學意義(P＞0.05);細胞超微結構觀察髮現染毒組細胞齣現粗麵內質網擴張.結論 二巰基敵枯雙可能是通過損傷甲狀腺濾泡細胞的粗麵內質網,從而影響甲狀腺毬蛋白的閤成與分泌;FRTL-5細胞培養液中甲狀腺毬蛋白的濃度可以作為體外篩選環境甲狀腺素榦擾物的敏感指標之一.
목적 관찰농약이구기활고쌍대FRTL-5세포주합성갑상선구단백적영향,병탐토기작용궤제이급FRTL-5세포주용우사선배경갑상선소간우물적가능성.방법 FRTL-5세포분별경0.1、1.0화10.0 μg/mL이구기활고쌍염독처리48 h후,용매조직화학법측정배양액중갑상선구단백적농도;용활세포파편적매세포화학염색관찰세포내갑상선과양화물매적표체강도,병용투사전경관찰최고농도처리조세포적초미결구.결과 수이구기활고쌍농도적증대,FRTL-5세포배양액중갑상선구단백적농도축점강저,기중0.1、1.0 μg/mL농도조여용제대조조상비차이유통계학의의(P＜0.05),10.0 μg/mL농도조배양액중미능검출갑상선구단백;매세포화학염색미견염독조세포갑상선과양화물매활성개변,도상분석결과 현시기양성세포적적분광밀도(IOD)치여대조조상비,차이무통계학의의(P＞0.05);세포초미결구관찰발현염독조세포출현조면내질망확장.결론 이구기활고쌍가능시통과손상갑상선려포세포적조면내질망,종이영향갑상선구단백적합성여분비;FRTL-5세포배양액중갑상선구단백적농도가이작위체외사선배경갑상선소간우물적민감지표지일.
Objective To study the impact of N' N-methylene-bis on thyroglobulin produced by FRTL-5 cells,and to explore the potential of using FRTL-5 cells to screen environmental thyroid hormone disruptors in vitro.Methods The FRTL-5 cells were treated with 0.1,1.0 and 10.0 μg/mL N'N-methylene-bis for 48 hours,respectively.The concentrations of thyroglobulin in the medium of the treated cells were detected by radioimmunoassay.The expression of thyroid peroxidases in the FRTL-5 cells was assessed by enzyme cytochemistry technique.The ultrastructure of the cells was also observed.Results The FRTL-5 cells treated with 0.1 and 1.0 μg/mL of N'N-methylene-bis produced less thyroglobulin than the controls(P＜0.05).No thyroglobulin was detected with the cells treated with 10.0 μg/mL of N'N-methylene-bis.No difference in the expression of thyroid peroxidases was found between the treated cells and the controls.The treated cells had expanded rough endoplasmic reticulum.Conclusion N' N-methylene-bis disrupts the bio-function of thyroid by damaging the rough endoplasmic reticulum of thyroid follicular cells.FRTL-5 cells can be used for screening thyroid hormone disruptors in vitro.
