CAJ | 학술논문

目的:探讨P16 mRNA和蛋白表达缺失与胃肠道间质瘤生物学行为及预后的关系.方法:采用反转录-聚合酶链式反应(RT-PCR)、免疫组织化学及Western Blot法分别检测胃肠道间质瘤组织中P16mRNA和蛋白表达情况,并对患者进行随访,分析P16表达水平与胃肠道间质瘤细胞核增殖指数、肿瘤细胞的侵袭危险性及胃肠道间质瘤患者生存预后间的关系,采用SPSS13.0统计软件包进行统计学分析.结果:胃肠道间质瘤中P16mRNA和蛋白的阳性表达率分别为53.8%(21/39,冰冻组织)、51.3%(20/39,冰冻组织)和51.4%(37/72,石蜡组织),不同侵袭危险性间比较差异有统计学显著性(P<0.05),随着侵袭危险性的增高,其表达率呈下降趋势;且与核增殖指数Ki-67表达及患者生存期呈负相关(P<0.05).结论:P16表达缺失与胃肠道间质瘤的浸润进展有关;P16mRNA和蛋白的检测有助于判断胃肠道间质瘤的生物学行为和预后.
목적:탐토P16 mRNA화단백표체결실여위장도간질류생물학행위급예후적관계.방법:채용반전록-취합매련식반응(RT-PCR)、면역조직화학급Western Blot법분별검측위장도간질류조직중P16mRNA화단백표체정황,병대환자진행수방,분석P16표체수평여위장도간질류세포핵증식지수、종류세포적침습위험성급위장도간질류환자생존예후간적관계,채용SPSS13.0통계연건포진행통계학분석.결과:위장도간질류중P16mRNA화단백적양성표체솔분별위53.8%(21/39,빙동조직)、51.3%(20/39,빙동조직)화51.4%(37/72,석사조직),불동침습위험성간비교차이유통계학현저성(P<0.05),수착침습위험성적증고,기표체솔정하강추세;차여핵증식지수Ki-67표체급환자생존기정부상관(P<0.05).결론:P16표체결실여위장도간질류적침윤진전유관;P16mRNA화단백적검측유조우판단위장도간질류적생물학행위화예후.
Objective: To investigate the relationship of loss of P16 expression with biologlical behaviors and prognosis of gastrointestinal stromal tumors(GISTs). Methods: The expression of P16 protein and mRNA was detected in GISTs tissues by immunohistochemistry,Western blot and RT-PCR.The prognosis was evaluated through follow up. Results: The expression rates of P16 protein and mRNA in GISTs were 53.8%(21/39，Frozen tissue)，51.3%(20/39，Frozen tissues)and 51.4%(37/72，Paraffin-embedded tissues)，respectively.The expression of P16 was significantly different among GISTs of different aggressive risk(P<0.05).With the incease of aggressive risk，the expression of P16 was deceased.The expression of P16 was negtively correlated with Ki-67 and patient survival(P<0.05). Conclusion: The loss of P16 expression has a positive correlation with the infiltration and progression of GIST.Detection of P16 protein and mRNA is helpful for the evalutaion of biological behaviors and prognosis of gastrointestinal stromal tumors.