CAJ | 학술논문

目的探讨运用导流杂交基因芯片技术(HybriMax)在临床人乳头瘤病毒(HPC)感染分型检测中的运用,调查重庆地区宫颈病变患者HPV病毒分型情况进行.方法采用导流杂交基因芯片技术,对473例宫颈病变患者以病理学诊断为金标准对宫颈病变进行分组后,进行生殖道HPV感染分型检测.结果 17种HPV亚型被检出,总检出率为63.0%(284/473),检出率较高的亚型为HPV16(23.7%、112/473)、HPV58(12.7%、60/473)、HPV53(7.4%、35/473).HPV感染率随宫颈病变SPI、CIN I、CIN II、CIN III的变化而升高(X2=77.06、P＜0.01).结论重庆地区最常见的HPV型别是HPV16、58型.导流杂交基因芯片技术能够时检测HR-HPV和LR-HPV,可判断多型感染;用于临床诊断和普查均为理想方法.
목적 탐토운용도류잡교기인심편기술(HybriMax)재림상인유두류병독(HPC)감염분형검측중적운용,조사중경지구궁경병변환자HPV병독분형정황진행.방법 채용도류잡교기인심편기술,대473례궁경병변환자이병이학진단위금표준대궁경병변진행분조후,진행생식도HPV감염분형검측.결과 17충HPV아형피검출,총검출솔위63.0%(284/473),검출솔교고적아형위HPV16(23.7%、112/473)、HPV58(12.7%、60/473)、HPV53(7.4%、35/473).HPV감염솔수궁경병변SPI、CIN I、CIN II、CIN III적변화이승고(X2=77.06、P＜0.01).결론 중경지구최상견적HPV형별시HPV16、58형.도류잡교기인심편기술능구시검측HR-HPV화LR-HPV,가판단다형감염;용우림상진단화보사균위이상방법.
Objective To evaluate the application of flow-through rapid hybridization technique and gene chip (HybriMax) on human papiUomavions (HPV) subtype in Chongqing.Methods Cervical tissue samples were taken under the colpnscope form 473 females who had cervical lesion for pathological analysis.The predictive value of HybriMax in cervical abnormality was compared with pathological results,which were used as golden standard.Resuits 13 different subtypes were found and total HPV positive rate was 63.0% (284/473) Among the 17 different subtypes ,the higher positive rote HPV subtypes were HPV16 (23.7%,112/473),HPV58 (12.7% ,60/473),HPV53(7.4% ,35/473).The HPV infection rates were higher with the worse d cervical lesion(X2=77.06,P＜0.01).Conclusions The most frequent subtypes of HPV infection in Chongqing cervical lesion were HPV 16,58.HybriMax was an effective method to detect HPV subtype in clinical.