CAJ | 학술논문

目的观察富血小板血浆(PRP)在新西兰大白兔皮肤缺损修复中的作用,探讨PRP促进软组织修复和皮肤愈合的疗效. 方法新西兰大白兔16只,体质量2.5～3.0 kg,雌雄不限.在背部脊柱两侧分别制作面积为3 cm×3 cm的全层皮肤缺损,随机选择任一侧为实验组,用PRP凝胶覆盖伤口(PRP组),对照组以自体全血凝胶覆盖伤口,随后分别用3M膜紧贴皮肤覆盖创面.分别于术后第5、10、15、20天通过大体和组织学观察来比较两组伤口愈合情况. 结果大体形态:术后第5天,对照组伤口可见炎性渗出物,2个伤口出现少量脓性分泌物,创面面积为(7.05±1.06)cm<'2>,PRP组伤口干燥,创面面积为(6.38±1.00)cm<'2>.术后第20天,PRP组创面完全闭合,对照组有2例表皮未完全覆盖伤口,创面面积为(0.20±0.24)cm<'2>.组织学观察:术后第5天,PRP组创面PRP凝块里修复细胞较多,而对照组自体血凝块中修复细胞较少.术后第20天,两组皮肤结构均较完整,但PRP组皮肤更显成熟,真皮层纤维排列较对照组整齐. 结论 PRP能有效地促进新西兰大白兔皮肤缺损的修复.
목적 관찰부혈소판혈장(PRP)재신서란대백토피부결손수복중적작용,탐토PRP촉진연조직수복화피부유합적료효. 방법 신서란대백토16지,체질량2.5～3.0 kg,자웅불한.재배부척주량측분별제작면적위3 cm×3 cm적전층피부결손,수궤선택임일측위실험조,용PRP응효복개상구(PRP조),대조조이자체전혈응효복개상구,수후분별용3M막긴첩피부복개창면.분별우술후제5、10、15、20천통과대체화조직학관찰래비교량조상구유합정황. 결과 대체형태:술후제5천,대조조상구가견염성삼출물,2개상구출현소량농성분비물,창면면적위(7.05±1.06)cm<'2>,PRP조상구간조,창면면적위(6.38±1.00)cm<'2>.술후제20천,PRP조창면완전폐합,대조조유2례표피미완전복개상구,창면면적위(0.20±0.24)cm<'2>.조직학관찰:술후제5천,PRP조창면PRP응괴리수복세포교다,이대조조자체혈응괴중수복세포교소.술후제20천,량조피부결구균교완정,단PRP조피부경현성숙,진피층섬유배렬교대조조정제. 결론 PRP능유효지촉진신서란대백토피부결손적수복.
Objective To observe the effects of platelet-rich plasma (PRP) on repairing skin defects in New Zealand rabbits. Methods Whole-thickness skin defects of 3 cm × 3 cm were created at the lef tand right sides of the back of 16 New Zealand rabbits, weighing 2. 5 to 3.0 kg. One side was randomly chosen as the experimental side, which was covered with PRP gel, while the other side was the control one, which was covered with blood gel. 3M film was used to cover the wounds postoperatively. After 5, 10, 15, and 20 days, gross and histological observations were performed to investigate the wound healing at the 2 sides.Results Inflammatory exudate from the wounds and a small amount of purulent exudate from 2 wounds were seen at the control sides 5 days after operation. The wounds at the PRP sides were dry in the same period.From 5 to 20 days, wounds at both sides contracted gradually, but more rapidly at PRP sides, with significant differences between the 2 groups. After 20 days, the wound skin at PRP sides was more mature than at the control sides. Conclusion PRP is effective in repairing skin defects, and can accelerate wound healing.