CAJ | 학술논문

目的探讨脾切除对脑缺血大鼠脑梗死体积的影响及其可能机制.方法 18只雄性Sprague- Dawley大鼠随机分为脾切除组、假脾切除术组和对照组,每组6只.脾切除2周后,采用线检法建立大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,24h后灌注固定并取脑.尼氏染色法测定脑梗死体积,免疫荧光染色法检测缺血皮质巨噬细胞数量.结果脾切除组(34.93％±3.23％)脑梗死体积显著小于假脾切除组(74.33％ +2.36％,q=39.399,P＜0.001)和对照组(77.30％±2.62％,q=42.369,P＜0.001),但假脾切除组与对照组之间无显著差异(q =2.970,p=0.082).脾切除组缺血皮质巨噬细胞数量[(3.4±1.07)个高倍镜视野]显著少于假脾切除组[(20.7±4.37)个/高倍镜视野,q=17.300,P＜0.001)和对照组[(18.87±4.17个)、高倍镜视野(q=15.467,P＜0.001];但假脾切除组与对照组无显著差异(q=1.833,P=0.384).结论脾切除可能通过减少脑缺血皮质区巨噬细胞数量缩小脑梗死体积.
목적 탐토비절제대뇌결혈대서뇌경사체적적영향급기가능궤제.방법 18지웅성Sprague- Dawley대서수궤분위비절제조、가비절제술조화대조조,매조6지.비절제2주후,채용선검법건립대서대뇌중동맥폐새(middle cerebral artery occlusion,MCAO)모형,24h후관주고정병취뇌.니씨염색법측정뇌경사체적,면역형광염색법검측결혈피질거서세포수량.결과 비절제조(34.93％±3.23％)뇌경사체적현저소우가비절제조(74.33％ +2.36％,q=39.399,P＜0.001)화대조조(77.30％±2.62％,q=42.369,P＜0.001),단가비절제조여대조조지간무현저차이(q =2.970,p=0.082).비절제조결혈피질거서세포수량[(3.4±1.07)개고배경시야]현저소우가비절제조[(20.7±4.37)개/고배경시야,q=17.300,P＜0.001)화대조조[(18.87±4.17개)、고배경시야(q=15.467,P＜0.001];단가비절제조여대조조무현저차이(q=1.833,P=0.384).결론 비절제가능통과감소뇌결혈피질구거서세포수량축소뇌경사체적.
Objective To investigate the effect of splenectomy on infarct volume in middle cerebral artery occlusion in focal cerebral ischemia rats and its possible mechanisms.Methods Eighteen male Sprague-Dawley rats were randomly divided into spleneetomy,sham splenectomy,and control groups (n =6 in each group).A model of middle cerebral artery occlusion (MCAO) was induced by the intraluminal suture method 2 weeks after spleneetomy.The rats were decapitated and their brains were removed after 24 hours.The infarct volume was measured with Nissl body staining The number of macrophages in ischemic cortex was detected with immunofluorescence staining Results The infarct volume in the splenectomy group (34.93％ + 3.23％ )was significantly smaller than that in the sham splenectomy group (74.33％ + 2.36％ ; q =39.399,P ＜ 0.001 ) and the control group (77.30％ + 2.62％ ; q =42.369,P ＜ 0.001 ).However,there was no significant difference between the sham splenectomy group and the control group (q =2.970,P =0.082).The number of macrophages of the ischemic cortex in the splenectomy group (3.4 ± 1.07/per high power field) was significantly less than that in the sham splenectomy group (20.7±4.37/per high power field; q =17.300,P＜0.001) and the control group (18.87 ±4.17/per high power field; q =15.467,P ＜0.001).However,there was no significant difference between the sham splenectomy group and the control goup (q =1.833,P =0.384).Conclusions Splenectomy may reduce the infarct volume by reducing the number of macrophages in ischemic corticalregion.