中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
10期
937-940
,共4页
冯帅南%徐祥%曾登芬%张猛%王莎莉%郭敏%简宇%张波%杨策%蒋建新%王正国%黄宏
馮帥南%徐祥%曾登芬%張猛%王莎莉%郭敏%簡宇%張波%楊策%蔣建新%王正國%黃宏
풍수남%서상%증등분%장맹%왕사리%곽민%간우%장파%양책%장건신%왕정국%황굉
伤口愈合%骨髓细胞%基质细胞衍生因子-1α
傷口愈閤%骨髓細胞%基質細胞衍生因子-1α
상구유합%골수세포%기질세포연생인자-1α
Wound healing%Bone marrow cells%Stromal cell derived factor-1α
目的 探讨皮肤创面基质细胞衍生因子-1α(stromal cell derived factor-1α,SDF-1α)在诱导骨髓来源细胞(bone marrow derived cells,BMDCs)向创面募集过程中的作用.方法分离培养小鼠BMDCs,并通过CXCR=4(SDF-1d特异性受体)免疫荧光鉴定BMDCs.培养的BMDC8分为正常培养组(BMDCs组)和CXCR4抗体共孵育组(100 ng/ml,anti-CXCR4组),用CM-DiI标记后分别将各组BMDC8注入全层皮肤缺损伤小鼠模型的尾静脉,同时以注射DMEM/F12无血清培养基作为对照组,观察创面标记的BMDCs数量及创面愈合率. 结果 (1)BMDCs均表达CXCR4;(2)在伤后7和14 d BMDCs组的创面愈合率分别为(41.3±4.6)%和(92.3±2.1)%,显著高于相应时相点对照组[(29.3±2.3)%、(77.3±2.5)%]和anti-CXCR4组[(30.7±4.6)%、(85.7±1.5)%](P＜0.05),而anti-CXCR4组的创面愈合率仅在伤后14 d显著高于对照组(P＜0.05);(3)在伤后7和14 d,BMDCs组创面标记的BMDCs数目分别为(535±84)个/每张切片和(769±124)个/每张切片,显著高于相应时相点的anti-CXCR4组[(335±97)个/每张切片;(521±127)个/每张切片](P＜0.05). 结论 BMDCs参与并促进皮肤创面愈合;SDF-1α在诱导BMDCs到达创面过程中有重要作用.
目的 探討皮膚創麵基質細胞衍生因子-1α(stromal cell derived factor-1α,SDF-1α)在誘導骨髓來源細胞(bone marrow derived cells,BMDCs)嚮創麵募集過程中的作用.方法分離培養小鼠BMDCs,併通過CXCR=4(SDF-1d特異性受體)免疫熒光鑒定BMDCs.培養的BMDC8分為正常培養組(BMDCs組)和CXCR4抗體共孵育組(100 ng/ml,anti-CXCR4組),用CM-DiI標記後分彆將各組BMDC8註入全層皮膚缺損傷小鼠模型的尾靜脈,同時以註射DMEM/F12無血清培養基作為對照組,觀察創麵標記的BMDCs數量及創麵愈閤率. 結果 (1)BMDCs均錶達CXCR4;(2)在傷後7和14 d BMDCs組的創麵愈閤率分彆為(41.3±4.6)%和(92.3±2.1)%,顯著高于相應時相點對照組[(29.3±2.3)%、(77.3±2.5)%]和anti-CXCR4組[(30.7±4.6)%、(85.7±1.5)%](P＜0.05),而anti-CXCR4組的創麵愈閤率僅在傷後14 d顯著高于對照組(P＜0.05);(3)在傷後7和14 d,BMDCs組創麵標記的BMDCs數目分彆為(535±84)箇/每張切片和(769±124)箇/每張切片,顯著高于相應時相點的anti-CXCR4組[(335±97)箇/每張切片;(521±127)箇/每張切片](P＜0.05). 結論 BMDCs參與併促進皮膚創麵愈閤;SDF-1α在誘導BMDCs到達創麵過程中有重要作用.
목적 탐토피부창면기질세포연생인자-1α(stromal cell derived factor-1α,SDF-1α)재유도골수래원세포(bone marrow derived cells,BMDCs)향창면모집과정중적작용.방법분리배양소서BMDCs,병통과CXCR=4(SDF-1d특이성수체)면역형광감정BMDCs.배양적BMDC8분위정상배양조(BMDCs조)화CXCR4항체공부육조(100 ng/ml,anti-CXCR4조),용CM-DiI표기후분별장각조BMDC8주입전층피부결손상소서모형적미정맥,동시이주사DMEM/F12무혈청배양기작위대조조,관찰창면표기적BMDCs수량급창면유합솔. 결과 (1)BMDCs균표체CXCR4;(2)재상후7화14 d BMDCs조적창면유합솔분별위(41.3±4.6)%화(92.3±2.1)%,현저고우상응시상점대조조[(29.3±2.3)%、(77.3±2.5)%]화anti-CXCR4조[(30.7±4.6)%、(85.7±1.5)%](P＜0.05),이anti-CXCR4조적창면유합솔부재상후14 d현저고우대조조(P＜0.05);(3)재상후7화14 d,BMDCs조창면표기적BMDCs수목분별위(535±84)개/매장절편화(769±124)개/매장절편,현저고우상응시상점적anti-CXCR4조[(335±97)개/매장절편;(521±127)개/매장절편](P＜0.05). 결론 BMDCs삼여병촉진피부창면유합;SDF-1α재유도BMDCs도체창면과정중유중요작용.
Objective To explore the effect of stromal cell-derived factor-1 α (SDF-1 α) on inducing recruitment of bone marrow-derived cells (BMDCs) to wound area. Methods BMDCs were isolated from bone marrow, cultured with routine method and identified by CXCR4 antibody. Cells cultured with CXCR4 antibody (100 ng,/mL) for 6 hours were labeled with CM-DiI and injected into the tail vein of full-thickness incisional wound model (set as anti-CXCR4 group). BMDCs labeled with CM-DiI without antibody treatment were injected to the rats in BMDCs group, and rats were injected with DMEM/F12 serum-free medium in the control group. The quantity of labeled BMDCs at the wound site and the percentage of wound closure were measured. Results (1) All BMDCs expressed CXCR4. (2) The percentages of wound closure at days 7 and 14 in BMDCs group (7 d: 41.3% ±4.6%; 14 d:92.3% ±2. 1%) were significantly higher than those of control group (7 d: 29.3% ±2. 3%; 14 d: 77.3% ±2.5%) and anti-CXCR4 group (7 d: 30.7% ±4.6% ;14 d: 85.7% ±1.5%) (P＜0.05). The percentage of wound closure of anti-CXCR4 group was significantly higher than that of control group at day 14(P ＜ 0.05). (3) The number of CM-DiI labeled BMDCs at wound site at days 7 and 14 in BMDCs group [7 d: (535 ±84) cells/hpf; 14 d: (769 ±124) cells/hpf) were greater than those of anti-CXCR4 group [7 d: (335 ±97) cells/hpf; 14 d: (521 ± 127) cells/hpf] (P＜0.05). Conclusions BMDCs participate in the cutaneous wound healing. SDF-1α plays an important role in recruiting BMDCs to wound area.
