CAJ | 학술논문

目的探讨血管内皮生长因子(VEGF)和转化生长因子-α(TGF-α)在尖锐湿疣(CA)血管形成中的作用.方法用RT-PCR和免疫组化法检测30例CA和15例正常包皮中VEGF、TGF-α和CD34表达.结果 CA中VEGF和TCF-α mRNA表达明显高于正常包皮(P<0.001);CA中VEGF、TGF-α蛋白阳性表达率分别为90%、86.7%,正常包皮中则为40%、26.7%,二者的差异具有统计学意义(P<0.01).CA中微血管密度比正常包皮明显增多(P<0.01),且CA中VEGF、TGF-α蛋白表达与MVD呈显著正相关(P<0.01).结论 CA中VEGF和TGF-α表达上调,可能促进血管生成,参与CA的发生、发展.
목적 탐토혈관내피생장인자(VEGF)화전화생장인자-α(TGF-α)재첨예습우(CA)혈관형성중적작용.방법 용RT-PCR화면역조화법검측30례CA화15례정상포피중VEGF、TGF-α화CD34표체.결과 CA중VEGF화TCF-α mRNA표체명현고우정상포피(P<0.001);CA중VEGF、TGF-α단백양성표체솔분별위90%、86.7%,정상포피중칙위40%、26.7%,이자적차이구유통계학의의(P<0.01).CA중미혈관밀도비정상포피명현증다(P<0.01),차CA중VEGF、TGF-α단백표체여MVD정현저정상관(P<0.01).결론 CA중VEGF화TGF-α표체상조,가능촉진혈관생성,삼여CA적발생、발전.
Objective To investigate the role of vascular endothelial growth factor (VEGF) and transforming growth factor-α (TGF-α) in the angiogenesis of condyloma acuminatum (CA). Methods Tissue specimens were obtained from the lesions of 30 patients with CA and foreskin of 15 normal human controls. Reverse transcription (RT)-PCR and immunohistochemical method were utilized to measure the protein and mRNA expressions of VEGF and TGF-α in the specimens. Microvessel density (MVD) was determined by staining with anti-CD34 polyclonal antibodies. Results A statistical elevation was observed in the expression of VEGF and TGF-α mRNA as well as MVD in CA specimens compared with the normal control specimens (P < 0.001, 0.001, 0.01). The expressions of VEGF and TGF-α protein were observed in 90% and 86.7% of the CA specimens, and in 40% and 26.7% of the control specimens (both P < 0.01). MVD was positively correlated with the expression of VEGF and TGF-α protein in CA tissues (both P < 0.01). Conclusion The overexpression of VEGF and TGF-α in CA tissue may accelerate angiogenesis in, and participate in the development and progression of, CA.