中国医药
中國醫藥
중국의약
CHINA MEDICINE
2010年
10期
907-909
,共3页
骨髓增生异常综合征%红细胞%天然免疫黏附功能%自然杀伤细胞
骨髓增生異常綜閤徵%紅細胞%天然免疫黏附功能%自然殺傷細胞
골수증생이상종합정%홍세포%천연면역점부공능%자연살상세포
Myelodysplastic syndromes%Erythrocyte%Innate immune adhering function%Natural killer cell
目的 探讨骨髓增生异常综合征患者红细胞天然免疫黏附功能及对自然杀伤细胞(NK细胞)杀伤活性的影响.方法 选择我院骨髓增生异常综合征患者14例为试验组,20例健康人作为对照组,将2组外周血红细胞用柠檬酸钠抗凝,检测红细胞在自身血浆条件下对K562细胞的免疫黏附并计算黏附率;并用四甲基偶氮唑盐微量酶反应比色(MTT)法测定红细胞对正常NK细胞杀伤K562细胞活性的影响,并与未加红细胞时进行比较.结果 试验组红细胞使K562细胞形成了"玫瑰花"样结合.对照组红细胞对K562细胞的免疫黏附结合率为(15.6±6.7)%,试验组红细胞对K562细胞的免疫黏附结合率为(8.5±7.0)%,2组比较差异有统计学意义(t=3.66,P＜0.01).不加红细胞条件下,对照组外周静脉血NK细胞杀伤K562细胞活性杀伤率为64%～75%,加入红细胞后为79%～88%;试验组不加入红细胞为45%～56%,加入红细胞后为51%～58%.加入红细胞后,2组NK红细胞对K562细胞的杀伤率均增加(P＜0.01),且对照组高于试验组(P＜0.01).结论 红细胞可增加NK细胞对K562细胞的杀伤率;骨髓增生异常综合征患者的红细胞对K562细胞的免疫黏附能力下降,并可减低NK细胞对K562细胞的杀伤率.
目的 探討骨髓增生異常綜閤徵患者紅細胞天然免疫黏附功能及對自然殺傷細胞(NK細胞)殺傷活性的影響.方法 選擇我院骨髓增生異常綜閤徵患者14例為試驗組,20例健康人作為對照組,將2組外週血紅細胞用檸檬痠鈉抗凝,檢測紅細胞在自身血漿條件下對K562細胞的免疫黏附併計算黏附率;併用四甲基偶氮唑鹽微量酶反應比色(MTT)法測定紅細胞對正常NK細胞殺傷K562細胞活性的影響,併與未加紅細胞時進行比較.結果 試驗組紅細胞使K562細胞形成瞭"玫瑰花"樣結閤.對照組紅細胞對K562細胞的免疫黏附結閤率為(15.6±6.7)%,試驗組紅細胞對K562細胞的免疫黏附結閤率為(8.5±7.0)%,2組比較差異有統計學意義(t=3.66,P＜0.01).不加紅細胞條件下,對照組外週靜脈血NK細胞殺傷K562細胞活性殺傷率為64%～75%,加入紅細胞後為79%～88%;試驗組不加入紅細胞為45%～56%,加入紅細胞後為51%～58%.加入紅細胞後,2組NK紅細胞對K562細胞的殺傷率均增加(P＜0.01),且對照組高于試驗組(P＜0.01).結論 紅細胞可增加NK細胞對K562細胞的殺傷率;骨髓增生異常綜閤徵患者的紅細胞對K562細胞的免疫黏附能力下降,併可減低NK細胞對K562細胞的殺傷率.
목적 탐토골수증생이상종합정환자홍세포천연면역점부공능급대자연살상세포(NK세포)살상활성적영향.방법 선택아원골수증생이상종합정환자14례위시험조,20례건강인작위대조조,장2조외주혈홍세포용저몽산납항응,검측홍세포재자신혈장조건하대K562세포적면역점부병계산점부솔;병용사갑기우담서염미량매반응비색(MTT)법측정홍세포대정상NK세포살상K562세포활성적영향,병여미가홍세포시진행비교.결과 시험조홍세포사K562세포형성료"매괴화"양결합.대조조홍세포대K562세포적면역점부결합솔위(15.6±6.7)%,시험조홍세포대K562세포적면역점부결합솔위(8.5±7.0)%,2조비교차이유통계학의의(t=3.66,P＜0.01).불가홍세포조건하,대조조외주정맥혈NK세포살상K562세포활성살상솔위64%～75%,가입홍세포후위79%～88%;시험조불가입홍세포위45%～56%,가입홍세포후위51%～58%.가입홍세포후,2조NK홍세포대K562세포적살상솔균증가(P＜0.01),차대조조고우시험조(P＜0.01).결론 홍세포가증가NK세포대K562세포적살상솔;골수증생이상종합정환자적홍세포대K562세포적면역점부능력하강,병가감저NK세포대K562세포적살상솔.
Objective To investigate the change of native immune adhering function (ENIAF) inself-plasma of patients with myelodysplastic syndrome and its effect on the killing activity of natural killer ( NK ) cells.Methods The whole blood was anticoagulated with citric acid. 5 μl precipitated red blood cells and 500 μl plasma of patients or controls were directly mixed with 750 μl quantitative K562 cells at 37℃ for 30 minutes. One K562 cell attached by one or more erythrocytes was counted as one rosette,the ratio of rosettes was calculated. Using K562 cells as target cells, the killing activity of NK cells isolated from normol persons was detected by MTT assay, the change of the killing activity was observed after adding RBCs. Results The ratio of rosettes formed by RBCs of 21 normal controls and K562 cells was (15.6 +6.7 )%, and the ratio of rosetfes formed by RBCs of 16 patients and K562 cells was (8.5 ±7.0)%. The ability of ENIAF in patients with myelodysplastic syndrome was significantly lower than that in healthy individuals( t = 3.66 ,P ＜ 0. 01 ). The killing rate of NK cells in peripheral blood of normal individuols was 64%-75% without adding RBCs, and it increased to 79%-88% after adding RBCs. The test group was 45%-56% without adding RBCs, and it increased to 51% -58% after adding RBCs. Conclusions It is concluded that the ENIAF of RBCs in patients with myelodysplastic syndrome decreases, accompanying the reduction of the killing activity of NK cells to K562 cells. To detect change of ENIAF may be helpful for the assessment of the immunological function of patients with myelodysplastic syndrome.
