CAJ | 학술논문

目的观察Rac1 mRNA及蛋白在神经胶质瘤组织中的表达情况,探讨Rac1与病理分级的相关性.方法应用免疫荧光组织化学、RT-PCR和Western blotting方法检测45例神经胶质瘤组织和10例正常脑组织标本中Rac1 mRNA及蛋白水平.结果 3种检测结果均表明,Rac1在正常脑组织中无表达,45例脑胶质瘤中最多42例有Rac1表达,表达率与病理分级呈正相关,即脑胶质瘤病理级别越高,Rac1阳性表达率越高.结论 Rac1在脑胶质瘤中的高表达与神经胶质瘤的侵袭、转移密切相关,可做为一个反映脑胶质瘤增殖能力和恶性程度的指标.
목적 관찰Rac1 mRNA급단백재신경효질류조직중적표체정황,탐토Rac1여병리분급적상관성.방법 응용면역형광조직화학、RT-PCR화Western blotting방법검측45례신경효질류조직화10례정상뇌조직표본중Rac1 mRNA급단백수평.결과 3충검측결과균표명,Rac1재정상뇌조직중무표체,45례뇌효질류중최다42례유Rac1표체,표체솔여병리분급정정상관,즉뇌효질류병리급별월고,Rac1양성표체솔월고.결론 Rac1재뇌효질류중적고표체여신경효질류적침습、전이밀절상관,가주위일개반영뇌효질류증식능력화악성정도적지표.
Objective To investigate the protein and mRNA levels of Rac1 in glioma tissues,and explore the correlation of Rac1 with pathological grades. Methods Immunofluorescence,RT-PCR and Western blotting were used to detect the protein and mRNA levels of Rac1 in 45 cases of gliomas tissues and 10 cases of normal brain tissues. Results The results indicated that normal brain tissues showed no protein and mRNA expressions of Rac1, and that of Rac1 highly expressed in glioma tissues (42/45 at most). The spearman correlation analysis revealed that the levels of transcription and expression of Rac1 were positively correlated to the tumor grades; positive expression rate of Rac1 in high grade of glioma was statistically higher than that in low grade of glioma. Conclusion The high expressions of Rac1 in the glioma are closely correlated to the tumor cell invasion and metastasis, which can be used as a marker indicating the malignance and proliferation of glioma.