CAJ | 학술논문

目的观察正常髓核细胞的黏弹性。方法髓核组织取材于3例脊柱侧凸矫形手术者术中取出废弃的髓核组织,用胰蛋白酶和Ⅱ型胶原酶消化分离细胞,Ⅱ型胶原免疫荧光组化和蕃红染色进行细胞鉴定,测量细胞直径,采用微管吸吮技术分析髓核细胞的黏弹性特性。结果髓核细胞直径为(15.40±1.83)μm,正常髓核细胞的黏弹性参数k1(0.101 ±0.052) kPn、k2(0.353±0.199) kPa和μ(3.034±1.843) kPa·s。直线相关性分析表明,仅k1与髓核细胞直径明显相关(r=-0.389,P＜0.05)。结论正常髓核细胞表现为典型的黏弹性固体蠕变特征;微管吸吮技术可以作为测量髓核细胞生物力学特性的可靠方法。
목적 관찰정상수핵세포적점탄성。방법 수핵조직취재우3례척주측철교형수술자술중취출폐기적수핵조직,용이단백매화Ⅱ형효원매소화분리세포,Ⅱ형효원면역형광조화화번홍염색진행세포감정,측량세포직경,채용미관흡전기술분석수핵세포적점탄성특성。결과 수핵세포직경위(15.40±1.83)μm,정상수핵세포적점탄성삼수k1(0.101 ±0.052) kPn、k2(0.353±0.199) kPa화μ(3.034±1.843) kPa·s。직선상관성분석표명,부k1여수핵세포직경명현상관(r=-0.389,P＜0.05)。결론 정상수핵세포표현위전형적점탄성고체연변특정;미관흡전기술가이작위측량수핵세포생물역학특성적가고방법。
Objective To study the viscoelastic properties of nucleus pulposus (NP) cells from human in vitro. Methods NP was obtained from discarded NP tissue of 3 scoliosis patients aged from 13 to 16 years. Pancreatin and collagenase type Ⅱ were used to digest NP and cells were isolated from NP. Type Ⅱ collagen immunofluorescence and Fan seaing were used to identify NP cells. The micropipette aspiration test was used in combination with a three-parameter viscoelastic solid model to measure the mechanical properties of NP cells. Results The mean diameter of the digested NP cells was ( 15.40 ± 1.83) μm. In response to a prescribed pressure, the NP cells exhibited viscolastic solid creep behavior, which was characterized initially by a jump in displacement followed by a monotony decreasing rate of deformation that generally reached an equilibrium. NP cells were deformed to a length as much as 2 times the radius of the micropipette without completely entering the micropipette. The viscolastic parameters were k1 (0. 101 ±0. 052) kPa, k2 (0. 353 ± 0. 199) kPa, and μ ( 3. 034 ± 1. 843 ) kPa· s, respectively. Only the k1 was positively correlated to the cell diameter (r =-0. 389, P ＜ 0. 05 ). Conclusion Human normal NP cells behave as a typical viscolastic solid creep. Micropipette aspiration technique is a valid method for the study on biomechanics of NP cells.