中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
8期
1153-1155,后插二
,共4页
常俊锴%魏云飞%冯宁翰%张炜
常俊鍇%魏雲飛%馮寧翰%張煒
상준개%위운비%풍저한%장위
半乳糖凝集素-3%邻苯二甲酸二丁酯%尿道下裂%生殖结节
半乳糖凝集素-3%鄰苯二甲痠二丁酯%尿道下裂%生殖結節
반유당응집소-3%린분이갑산이정지%뇨도하렬%생식결절
Galectin-3%Di-n-butyl phthalate%Hypospadias%Genital tubercle
目的 利用邻苯二甲酸二丁酯(DBP)胚胎期暴露导致SD大鼠尿道下裂发生的动物模型,观察半乳糖凝集素-3(galectin-3)在尿道下裂与正常大鼠生殖结节中的差异表达,探讨其在DBP致大鼠尿道下裂发生中的作用.方法 SD孕鼠20只,随机分为2组,妊娠14～18 d(GD14～18)实验组和对照组分别给予DBP 800 mg/(kg·d)和大豆油2 ml/d灌胃,GD19剖宫产后统计子鼠数、雄性子鼠出生体质量(BW)及肛门生殖器距离(AGD)、尿道下裂发生率,取两组雄性子鼠生殖结节,运用免疫印迹法和免疫组织化学方法分析galectin-3的表达.结果 尿道下裂仅实验组发生,发生率为40%,每窝子鼠数、雄性子鼠BW及AGD/BW在实验组分别为(8.90±1.25)只、(5.12±0.24)g、(0.57±0.03)mm/g,在对照组分别为(13.20±1.46)只、(6.72±0.42)g、(0.66±0.07)mm/g,差异有统计学意义(P＜0.05);galectin-3在尿道下裂组的相对表达量为0.603±0.014(n=10),正常对照组为0.851±0.015(n=10),差异有统计学意义(P＜0.05);galectin-3主要定位于生殖结节上皮细胞,尿道下裂组染色强度明显弱于对照组.结论 染毒期间DBP对雄性子鼠有明显毒性作用,改变了生殖结节中galectin-3的表达,影响了上皮细胞增殖、凋亡及尿生殖褶的融合.
目的 利用鄰苯二甲痠二丁酯(DBP)胚胎期暴露導緻SD大鼠尿道下裂髮生的動物模型,觀察半乳糖凝集素-3(galectin-3)在尿道下裂與正常大鼠生殖結節中的差異錶達,探討其在DBP緻大鼠尿道下裂髮生中的作用.方法 SD孕鼠20隻,隨機分為2組,妊娠14～18 d(GD14～18)實驗組和對照組分彆給予DBP 800 mg/(kg·d)和大豆油2 ml/d灌胃,GD19剖宮產後統計子鼠數、雄性子鼠齣生體質量(BW)及肛門生殖器距離(AGD)、尿道下裂髮生率,取兩組雄性子鼠生殖結節,運用免疫印跡法和免疫組織化學方法分析galectin-3的錶達.結果 尿道下裂僅實驗組髮生,髮生率為40%,每窩子鼠數、雄性子鼠BW及AGD/BW在實驗組分彆為(8.90±1.25)隻、(5.12±0.24)g、(0.57±0.03)mm/g,在對照組分彆為(13.20±1.46)隻、(6.72±0.42)g、(0.66±0.07)mm/g,差異有統計學意義(P＜0.05);galectin-3在尿道下裂組的相對錶達量為0.603±0.014(n=10),正常對照組為0.851±0.015(n=10),差異有統計學意義(P＜0.05);galectin-3主要定位于生殖結節上皮細胞,尿道下裂組染色彊度明顯弱于對照組.結論 染毒期間DBP對雄性子鼠有明顯毒性作用,改變瞭生殖結節中galectin-3的錶達,影響瞭上皮細胞增殖、凋亡及尿生殖褶的融閤.
목적 이용린분이갑산이정지(DBP)배태기폭로도치SD대서뇨도하렬발생적동물모형,관찰반유당응집소-3(galectin-3)재뇨도하렬여정상대서생식결절중적차이표체,탐토기재DBP치대서뇨도하렬발생중적작용.방법 SD잉서20지,수궤분위2조,임신14～18 d(GD14～18)실험조화대조조분별급여DBP 800 mg/(kg·d)화대두유2 ml/d관위,GD19부궁산후통계자서수、웅성자서출생체질량(BW)급항문생식기거리(AGD)、뇨도하렬발생솔,취량조웅성자서생식결절,운용면역인적법화면역조직화학방법분석galectin-3적표체.결과 뇨도하렬부실험조발생,발생솔위40%,매와자서수、웅성자서BW급AGD/BW재실험조분별위(8.90±1.25)지、(5.12±0.24)g、(0.57±0.03)mm/g,재대조조분별위(13.20±1.46)지、(6.72±0.42)g、(0.66±0.07)mm/g,차이유통계학의의(P＜0.05);galectin-3재뇨도하렬조적상대표체량위0.603±0.014(n=10),정상대조조위0.851±0.015(n=10),차이유통계학의의(P＜0.05);galectin-3주요정위우생식결절상피세포,뇨도하렬조염색강도명현약우대조조.결론 염독기간DBP대웅성자서유명현독성작용,개변료생식결절중galectin-3적표체,영향료상피세포증식、조망급뇨생식습적융합.
Objective To verify the differential expression of galectin-3 in the genital tubercle (GT) of hypospadiac rats induced by maternal exposure to Di-n-butyl phthalate (DBP) and normal control rats in order to further explore the mechanism of hypospadias. Methods Twenty pregnant SD rats were randomly divided into two groups which were given DBP and soybean oil gavage at the dose of 800 mg/(kg·d) and 2 ml/d separately during the 14-18th day of pregnancy. Fetal rats per fossa, birth weight ( BW) , ano-genital distance (AGD), hapospadias incidence, and harvested GT of fetal rats at GD19.The expression of galectin-3 was analyzed by Western blotting and immunohistochemistry. Results The incidence of hypospadias was 40% , the rat offspring per litter in experiment group was significantly less than in control group (P < 0.05), (experiment group of 8.90 ± 1.25, control group of 13.20 ± 1.46) , BW and AGD/ BW of male offspring in control group were (6.72 ±0.42) g, (0.66 ±0.07) mra/g, those in experiment group were (5.12 ±0.24) g, (0.57 ±0.03) mm/g, and the expression of galectin-3 had statistically significant difference (P < 0.05). Galectin-3 was mainly located in the cytoplasm of epithelium, and staining intensity in control group was stronger than in experimental group. Conclusion Maternal exposure to DBP can reduce the galectin-3 expression in GT epithelium, affect cellular proliferation, apoptosis and urethral fold fusion, and finally result in the occurrence of hypospadias.
