CAJ | 학술논문

帕金森病是一种常见的中枢神经系统进行性退行性病变,其病因机制尚未明确,且缺乏令人满意的治疗方法.因此,建立有效的帕金森病模型对其病因机制的进一步探讨有着重要的意义.Berger等人于1982年建立腹侧中脑原代细胞培养方法,通常应用小鼠或大鼠胚胎(13～15 d胎龄)中脑组织进行培养.多巴胺能神经元可在体外存活长达数月,因此,可为多巴胺能神经元急性或慢性损伤的病因机制的研究提供实验手段.多巴胺能神经元在体外可通过免疫化学、放射自显影及荧光组织化学等方法确认.故其形态学及数量上的改变在体外较容易被检测,因而这种实验模型的建立对于帕金森病病因机制及新的治疗方法的探讨将起着重要的作用.
파금삼병시일충상견적중추신경계통진행성퇴행성병변,기병인궤제상미명학,차결핍령인만의적치료방법.인차,건립유효적파금삼병모형대기병인궤제적진일보탐토유착중요적의의.Berger등인우1982년건립복측중뇌원대세포배양방법,통상응용소서혹대서배태(13～15 d태령)중뇌조직진행배양.다파알능신경원가재체외존활장체수월,인차,가위다파알능신경원급성혹만성손상적병인궤제적연구제공실험수단.다파알능신경원재체외가통과면역화학、방사자현영급형광조직화학등방법학인.고기형태학급수량상적개변재체외교용역피검측,인이저충실험모형적건립대우파금삼병병인궤제급신적치료방법적탐토장기착중요적작용.
Parkinson's disease (PD) is a progressive degenerative disorder of the central nervous system. The etiology and molecular mechanism of PD remain unclear. Though effective treatment exists for most cases at its onset, the symptoms become resistant as time goes on and the underlying neurodegeneration cannot be stopped. Therefore, it is very necessary to establish animal and cell culture models reflecting the pathological changes and the efficacy of treatment. Next to neurotoxic studies with MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) in monkeys and mice, cell cultures of dissociated fetal nigral tissue including dopaminergic cells of the mesencephalon are currently used. The technique of preparing primary culture of ventral mesencephalon was put forward by Berger and his colleagues in 1982. Dissociated ventral mesencephalic tissue from mice or rat embryos at gestation day 13 to 15 usually was used to make primary cell culture in which the dopaminergic neurons can be maintained for several months. So such a long term culture system can be developed in vitro permits studies on both acute and chronic lesions in dopaminergic neurons. Radioligands, fluorescence microscopy and immunohistochemistry for tyrosine hydroxylase have been used to identify dopaminergic neurons. Changes in morphology and loss of the dopaminergic neurons can be easily detected, so this cell model will play an important role in the research of etiology, mechanism and novel treatments of PD.