CAJ | 학술논문

目的探索从新生小鼠脂肪组织中分离纯化雪旺细胞的可行性.方法取新生(出生5～7 d)绿色荧光蛋白(GFP)转基因小鼠的腹股沟皮下脂肪,采用0.2%的复合胶原酶NB4消化法分离获取细胞,然后用含10%胎牛血清(FBS)的低糖DMEM培养基培养.用0.1%的复合胶原酶NB4差速分离纯化雪旺细胞,每48 h纯化1次,共进行2次纯化.用p75免疫荧光法鉴定雪旺细胞并分析其纯度.结果从小鼠腹股沟皮下脂肪中分离纯化获得的细胞是雪旺细胞,经过2次纯化,其纯度可达90%.结论从小鼠脂肪组织中可以分离纯化获得雪旺细胞,为组织工程人工神经种子细胞提供一种新的来源.
목적 탐색종신생소서지방조직중분리순화설왕세포적가행성.방법 취신생(출생5～7 d)록색형광단백(GFP)전기인소서적복고구피하지방,채용0.2%적복합효원매NB4소화법분리획취세포,연후용함10%태우혈청(FBS)적저당DMEM배양기배양.용0.1%적복합효원매NB4차속분리순화설왕세포,매48 h순화1차,공진행2차순화.용p75면역형광법감정설왕세포병분석기순도.결과 종소서복고구피하지방중분리순화획득적세포시설왕세포,경과2차순화,기순도가체90%.결론 종소서지방조직중가이분리순화획득설왕세포,위조직공정인공신경충자세포제공일충신적래원.
Objective To explore the possibility of isolating and purifying Schwann cells from newborn mouse fat tissues. Methods Adipose tissue was harvested from 5 to 7 day-old neonatal GFP mice. The tissue was digested with 0.2% compound collagenase NB4 to obtain cells. The derived cells were then cultured in lowglucose DMEM with 10% FBS for 24 hours. 0.1% compound collagenase NB4 was used to purify Schwann cells by differential isolation once every 48 hours for two times total. p75 fluorescent immunostaining was performed to determine the purity of Schwann cells. Results The cells isolated from mouse groin subcutaneous fat tissue were Schwann cells. The purity of Schwann cells reached as high as 90% after two time purification.Conclusion Purified Schwann cells can be isolated from mouse fat tissue, which provides a new source for seed cells for tissue-engineered nerves.