CAJ | 학술논문

目的观察中国2型糖尿病人群中内皮细胞一氧化氮合酶(eNOS)基因多态性位点rs1799983与rs2070744是否与冠心病风险相关.方法以2005年3月至2010年10月412例2型糖尿病胸痛患者为研究人群,其中266例患有冠心病[男性171例,女性95例,平均年龄(64±9岁)],146例为无冠心病对照[男性67例,女性79例,平均年龄(62±10)岁].选取eNOS的2个单核苷酸多态性(SNPs):rs1799983(G＞T)和rs2070744(T＞C).应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术对eNOS基因SNPs读取个体基因型,采用病例-对照研究的方法,研究eNOS基因多态性与冠心病风险的关系.结果 SNP rs1799983在冠心病组与无冠心病组中T等位基因频率分别为10.4％和9.3％,T等位基因携带者较非携带者冠心病风险有增高趋势(OR=1.214),但差异无统计学意义(P＞0.05).SNP rs2070744在冠心病组与无冠心病组中C等位基因频率分别为10.5％和9.4％,C等位基因携带者较非携带者冠心病风险有增加趋势(OR=1.117),但差异亦无统计学意义(P＞0.05).校正其他已知冠心病风险因素如性别、年龄、BMI、糖尿病病程、高血压病病史、脂代谢异常病史以及吸烟史后,仍未发现rs1799983和rs2070744与冠心病风险的相关性.结论在中国2型糖尿病人群中,eNOS基因SNP rs1799983和rs2070744可能不是冠心病的主要风险因子.
목적 관찰중국2형당뇨병인군중내피세포일양화담합매(eNOS)기인다태성위점rs1799983여rs2070744시부여관심병풍험상관.방법 이2005년3월지2010년10월412례2형당뇨병흉통환자위연구인군,기중266례환유관심병[남성171례,녀성95례,평균년령(64±9세)],146례위무관심병대조[남성67례,녀성79례,평균년령(62±10)세].선취eNOS적2개단핵감산다태성(SNPs):rs1799983(G＞T)화rs2070744(T＞C).응용취합매련반응-한제성내절매편단장도다태성(PCR-RFLP)기술대eNOS기인SNPs독취개체기인형,채용병례-대조연구적방법,연구eNOS기인다태성여관심병풍험적관계.결과 SNP rs1799983재관심병조여무관심병조중T등위기인빈솔분별위10.4％화9.3％,T등위기인휴대자교비휴대자관심병풍험유증고추세(OR=1.214),단차이무통계학의의(P＞0.05).SNP rs2070744재관심병조여무관심병조중C등위기인빈솔분별위10.5％화9.4％,C등위기인휴대자교비휴대자관심병풍험유증가추세(OR=1.117),단차이역무통계학의의(P＞0.05).교정기타이지관심병풍험인소여성별、년령、BMI、당뇨병병정、고혈압병병사、지대사이상병사이급흡연사후,잉미발현rs1799983화rs2070744여관심병풍험적상관성.결론 재중국2형당뇨병인군중,eNOS기인SNP rs1799983화rs2070744가능불시관심병적주요풍험인자.
Objective To investigate the association of the genetic polymorphisms at endothelial nitric oxide synthase (eNOS) locus with the risk of coronary artery disease (CAD) in type 2 diabetics in China.Methods A total of 412 type 2 diabetics with chest pain treated from March 2005 to October 2010were included in this study,of whom 266 individuals with coronary artery disease were enrolled in group CAD ( 171 males and 95 females,average age (64 ±9 )yrs) and 146 cases without CAD were set as controls (67 males and 79 females,average age (62 ± 10 ) yrs). Two haplotype-tagging single nucleotide polymorphisms (SNPs) in eNOS gene were selected: rs1799983 (G ＞ T) and rs2070744 (T ＞ C).Genotypes of the 2 SNPs were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.The frequencies of genotypes and alleles at the loci were compared between the two groups.Results The frequencies of allele T at SNP rs1799983 were 10.4％ in the group CAD and 9.3％ in the controls,respectively.The carriers of allele T at rs1799983 had a tendency with a higher risk of CAD compared to non-carriers ( OR =1.214,but P ＞ 0.05 ).The frequency of allele C at rs2070744 was 10.5％ in the group CAD and 9.4％ in the controls.The carriers of allele C at rs2070744 had a higher risk of CAD compared to non-carriers ( OR =1.117,but P ＞ 0.05 ).No significant associations of the 2 SNPs with CAD risk was found in the studied population even after adjusted for the other known CAD risk factors,such as gender,age,body mass index,course of diabetes,history of hypertension and lipid metabolism disorders.Conclusion It suggests that the rs1799983 and rs2070744 at eNOS locus may not be the key risk factors for CAD in the Chinese patients with type 2 diabetes mellitus.