CAJ | 학술논문

目的快速重编程羊水细胞为多能干细胞,建立诱导分化研究的内对照模型.方法应用逆转录病毒介导4种基因(Oct4、Sox2、c-Myc、Klf4)诱导双胎羊水细胞,筛选羊水诱导性多能干细胞(human amniotic fluid-derived cells induced pluripotent stem cells,hAFDCs-iPSCs),对其进行多能性、体内外分化能力、核型等鉴定.结果双胎hAFDC-iPSCs能维持自我更新状态,在蛋白和mRNA水平上高表达全能性的标志基因,具有体内外分化潜能,在体外长期培养能维持正常核型.结论双胎hAFDCs-iPSCs遗传背景相一致,可以为iPSCs的诱导分化研究提供很好的内对照模型,并为胎儿的细胞自体移植治疗提供理想来源.
목적 쾌속중편정양수세포위다능간세포,건립유도분화연구적내대조모형.방법 응용역전록병독개도4충기인(Oct4、Sox2、c-Myc、Klf4)유도쌍태양수세포,사선양수유도성다능간세포(human amniotic fluid-derived cells induced pluripotent stem cells,hAFDCs-iPSCs),대기진행다능성、체내외분화능력、핵형등감정.결과 쌍태hAFDC-iPSCs능유지자아경신상태,재단백화mRNA수평상고표체전능성적표지기인,구유체내외분화잠능,재체외장기배양능유지정상핵형.결론 쌍태hAFDCs-iPSCs유전배경상일치,가이위iPSCs적유도분화연구제공흔호적내대조모형,병위태인적세포자체이식치료제공이상래원.
Objective To reprogram amniotic fluid cells into pluripotent stem cells in order to create an optimal internal control model for directed cell differentiation.Methods Human amniotic fluid-derived cells (hAFDCs) from heterozygotic twin fetuses were induced by retroviral vectors encoding Oct4,Sox2,c-Myc and Klf4.In vivo pluripotency,differentiation capacity and karyotype of hAFDCs induced pluripotent stem cells (hAFDCs-iPSCs) were determined.Results hAFDC-iPSCs derived from heterozygotic twins have maintained self renewal,with expression of high pluripotency marker gene detected at both mRNA and protein levels.The cells have maintained their differentiation capacity both in vitro and vivo,and showed normal karyotypes after long-term culturing in vitro. Conclusion hAFDCs-iPSCs derived from heterozygotic twins have good consistency in terms of genetic background,and can provide a good internal control for directed differentiation of iPSCs,and may be used an ideal source for autologous cell replacement therapy in the later life of the fetus.