CAJ | 학술논문

新鲜牦牛肝匀浆、磷酸缓冲液抽提、硫酸铵分级沉淀,并采用响应面法对提取条件进行优化.在最佳提取条件下提取的粗蛋白经DEAE-52离子交换层析和Sephadex G-200凝胶柱洗脱得到7种不同的蛋白.用SDS聚丙烯酰胺凝胶电泳对各蛋白的分析结果,各蛋白均成单一条带表明纯化效果较好,其分子量分布在97.2 KD-116 KD之间.采用化学发光法对其在体外抗氧化活性研究,主要包括超氧阴离子自由基、羟自由基、1,1-二苯基-2-三硝基苯肼自由基的清除实验,结果表明几种蛋白均具有抗氧化作用,尤其蛋白5和蛋白1作用较为突出,在250mg/L浓度时,对超氧阴离子的清除率为82.91％和72.92％.上述结果为牦牛肝作为抗氧化保健食品或功能食品的研究和开发提供了依据.
신선모우간균장、린산완충액추제、류산안분급침정,병채용향응면법대제취조건진행우화.재최가제취조건하제취적조단백경DEAE-52리자교환층석화Sephadex G-200응효주세탈득도7충불동적단백.용SDS취병희선알응효전영대각단백적분석결과,각단백균성단일조대표명순화효과교호,기분자량분포재97.2 KD-116 KD지간.채용화학발광법대기재체외항양화활성연구,주요포괄초양음리자자유기、간자유기、1,1-이분기-2-삼초기분정자유기적청제실험,결과표명궤충단백균구유항양화작용,우기단백5화단백1작용교위돌출,재250mg/L농도시,대초양음리자적청제솔위82.91％화72.92％.상술결과위모우간작위항양화보건식품혹공능식품적연구화개발제공료의거.
Seven proteins ( BGP1,BGP2,BGP3,BGP4,BGP5,GBP6,and BGP7) were isolated respectively from the liver of yak (Bos grunniens) by homogenizing,step precipitating with ammonium sulfate,ion-exchange chromatography on DEAE-52 and gel filtration chromatography on Sepbhadex G-200.Each protein gave a single band on SDS-PAGE and the molecular weight of those proteins were from 97.2 KD to 116 KD.The anti-oxidant activities of proteins were evaluated using the method of chemistry luminescence,including 1,1-dipheny1-2-picrylhydrazyl (DPPH ·),superoxide (O2(..))and hydroxyl (· OH) radical scavenging assays.The proteins could scavenge superoxide radical,hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH ·).Especially,the effects of BGP5 and BGP1 on scavenging superoxide free radical were obvious,with scavenging ability 82.91％ and 72.92％ at the concentrations of 250 mg/L.The results indicated that proteins may be exploited as health food or functional food.