CAJ | 학술논문

目的探讨胶质细胞生长因子(GGF)、神经生长因子(NGF)与垂体腺瘤临床生物学特性的关系。对术前、术后生长作预测。方法免疫组织化学法分析GGF、NGF在86例泌乳素垂体瘤(PRL)中的表达，分析GGF、NGF的表达与PRL水平、垂体腺瘤微血管密度、侵袭性及卒中等的关系。用GGF、NGF分别干预不同阶段的PRL细胞，观察细胞生长、周期变化及血管生成情况。通过相关分析及卡方检验证明肿瘤生长速率与GGF、NGF有无相关性。结果GGF在卒中组、侵袭性垂体腺瘤、复发PRL中均强阳性表达(P＜0.05)，微血管密度明显升高(P＜0.01)。NGF在卒中组、侵袭性垂体腺瘤、高泌乳素水平垂体腺瘤中的表达明显低于非卒中组、非侵袭性垂体腺瘤、低泌乳素水平的垂体腺瘤(P＜0.05)；在NGF作用下，微血管密度明显降低(P＜0.05)。GGF均与生长速率呈显著正相关，而NGF与PRL卒中、侵袭性呈负相关。结论GGF是促进PRL生长及侵袭性的重要因素之一，NGF能部分抑制PRL细胞的增殖。2者能间接反映PRL的增殖活性，为临床上评价PRL侵袭性、复发、治疗及预后提供重要的参考指标。
목적 탐토효질세포생장인자(GGF)、신경생장인자(NGF)여수체선류림상생물학특성적관계。대술전、술후생장작예측。방법면역조직화학법분석GGF、NGF재86례비유소수체류(PRL)중적표체，분석GGF、NGF적표체여PRL수평、수체선류미혈관밀도、침습성급졸중등적관계。용GGF、NGF분별간예불동계단적PRL세포，관찰세포생장、주기변화급혈관생성정황。통과상관분석급잡방검험증명종류생장속솔여GGF、NGF유무상관성。결과GGF재졸중조、침습성수체선류、복발PRL중균강양성표체(P＜0.05)，미혈관밀도명현승고(P＜0.01)。NGF재졸중조、침습성수체선류、고비유소수평수체선류중적표체명현저우비졸중조、비침습성수체선류、저비유소수평적수체선류(P＜0.05)；재NGF작용하，미혈관밀도명현강저(P＜0.05)。GGF균여생장속솔정현저정상관，이NGF여PRL졸중、침습성정부상관。결론GGF시촉진PRL생장급침습성적중요인소지일，NGF능부분억제PRL세포적증식。2자능간접반영PRL적증식활성，위림상상평개PRL침습성、복발、치료급예후제공중요적삼고지표。
Objective To explore the relationship between glial growth factor (GGF), nerve growth factor (NGF) and growth characteristics of prolactinoma (PRL) and to evaluate pre and postoperative growth of PRL. Methods Immunohistochemistry was used to analyze expression of GGF and NGF in 86 cases of PRL and to analyze the relationship between expression of GGF, NGF and PRL level, invasion, stroke, microvessel density. Cells were cultured with GGF and NGF to observe cell growth, cell cycle and angiogenesis. The relationship between proliferation, growth rate and GGF, NGF was evaluated by rank correlation and Chi-square test. Results GGF expression was significantly higher in invasive, stroke and recurrent pituitary adenomas ( P ＜ 0.05 ).Microvessel density increased significantly ( P ＜ 0.01 ). NGF expression was significantly lower in invasive, apoplexy and recurrent adenomas ( P ＜ 0. 05 ). Microvessel density decreased dramatically with NGF interruption ( P ＜ 0.05 ). GGF showed a positive correlation with growth rate of PRL. NGF showed a negative correlation with invasion and stroke. Conclusions GGF is one of the factors facilitating growth and invasion of PRL while NGF can partly restrain proliferation of PRL cells. Expression of GGF and NGF indirectly reflects proliferation activity of PRL and can be used as markers to evaluate invasion, recurrence, treatment response and prognosis of PRL.