CAJ | 학술논문

目的寻找一种既能抑制胶质瘤新生血管的形成,又能杀灭胶质瘤细胞的细胞因子,为临床肿瘤药物的筛选提供理论依据.方法培养传代大鼠C6胶质瘤细胞株以每孔1×10~4个细胞铺96孔培养板,分组加入AG3340[金属蛋白酶(MMPs)非肽类抑制剂,终浓度分别为O ng/ml、50 ng/ml、100ng/ml、150ng/ml],培养48 h后,弃去培养液,每孔加入DMSO(二甲亚砜)150μl,振荡后用酶标仪570nm 测定吸光度值(A值).结果 (1)随着加入AG3340浓度增加,部分大鼠C6胶质瘤细胞便会出现变圆、离壁、浮起直至死亡的现象.(2)对照组OD(取相同倍数视野中心)值:0.1645±0.015;低浓度组OD值:0.1443±0.011;中浓度组OD值:0.1358±0.011;高浓度组OD值:0.1213±0.014.总体及各实验组间比较,均有统计学意义(总体F=46.67,P<0.01;各组间P<0.05).结论 AG3340对大鼠C6胶质瘤细胞增殖有明显的抑制作用,且与浓度呈一定相关性,随着浓度的增加其抑制作用逐渐增强.
목적 심조일충기능억제효질류신생혈관적형성,우능살멸효질류세포적세포인자,위림상종류약물적사선제공이론의거.방법 배양전대대서C6효질류세포주이매공1×10~4개세포포96공배양판,분조가입AG3340[금속단백매(MMPs)비태류억제제,종농도분별위O ng/ml、50 ng/ml、100ng/ml、150ng/ml],배양48 h후,기거배양액,매공가입DMSO(이갑아풍)150μl,진탕후용매표의570nm 측정흡광도치(A치).결과 (1)수착가입AG3340농도증가,부분대서C6효질류세포편회출현변원、리벽、부기직지사망적현상.(2)대조조OD(취상동배수시야중심)치:0.1645±0.015;저농도조OD치:0.1443±0.011;중농도조OD치:0.1358±0.011;고농도조OD치:0.1213±0.014.총체급각실험조간비교,균유통계학의의(총체F=46.67,P<0.01;각조간P<0.05).결론 AG3340대대서C6효질류세포증식유명현적억제작용,차여농도정일정상관성,수착농도적증가기억제작용축점증강.
Objective To find a cellular factor that can inhibit the formation of tumor angiogenesis, and kill tumor cells, which provide a theoretical basis for clinical oncology drug screen. Methods Delivered C6 rat glioma cells ( purchased from Shanghai cells institute) into single-cell suspensions with Dropper as each hole had 1 ×10~4 cells. Resurface 96-well culture plates. Added AG3340 containing different concentrations, then discarded the medium, and then added each hole DMSO150μl. After oscillating, the magnitude of absorbance (A value) was measured with microplate reader (Bio-Rad Model 550 USA). Results (1) Compared with the control group, AG3340 could inhibit cell proliferation. It had a gradual better effect as the concentrations increased, showing a dose-dependent character. When exposed in a higher concentration of AG3340, some of C6 rat glioma cells appeared rounded, slide away from the wall and float up, even to the phenomenon of death. (2) Between the experimental group (50 ng/ ml group OD value: 0.1426 ±0.022; 100ng/ml group OD value: 0.1390 ±0.007;150ng/ ml group OD value;0.1226 ±0.006) with the control group (OD value;0.165 ±0.101) , as well as various experimental groups. There was statistically significant (P<0.05) by the Student-Newman-Keul test. Conclusions Inhibitory effect of AG3340 in the proliferation of C6 rat glioma cells is obvious and dose-dependent.