中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
11期
1012-1016
,共5页
梁惠萍%吴继周%吴健林%韦颖华%万裴琦%吴霜%龚星光%孟艳丽%罗双艳%虞艳琦
樑惠萍%吳繼週%吳健林%韋穎華%萬裴琦%吳霜%龔星光%孟豔麗%囉雙豔%虞豔琦
량혜평%오계주%오건림%위영화%만배기%오상%공성광%맹염려%라쌍염%우염기
HLA-DRB1*12%IL-4%IFN-γ%乙肝疫苗%免疫应答
HLA-DRB1*12%IL-4%IFN-γ%乙肝疫苗%免疫應答
HLA-DRB1*12%IL-4%IFN-γ%을간역묘%면역응답
HLA-DRB1 * 12%IL-4%IFN-γ%Hepatitis B vaccination%Immune response
目的 探讨人群HLA-DRB1*12基因及相应位点下IL-4及IFN-γ对乙肝疫苗免疫应答的影响.方法 选取74名对乙肝疫苗无、弱应答的广西籍健康大学生及条件相近的64名中、强应答者,采用PCR-SSP技术检测受试者的HLA-DRB1* 12等位基因,采用ELISA法检测血清中IFN-γ水平及IL-4因子水平.结果 (1)无、弱应答组的HLA-DRB1*12等位基因表达频率为10.8％,低于中、强应答组的32.8％ (P=0.002)；(2)无、弱应答组的IFN-γ平均表达水平为(7.21±7.92) ng/ml,显著低于中、强应答组(16.36±11.00) ng/ml( P=0.000)；(3)无、弱应答组中的IL-4平均表达水平为(3.18±4.45) ng/ml,显著低于中、强应答组(7.76±5.71) ng/ml(P=0.000)；(4) HLA-DRB1* 12阳性组IFN-γ平均表达水平为(13.18±11.24) ng/ml,阴性组平均表达水平为(11.00±10.29) ng/ml,两组间差异无统计学意义(P=0.349)；(5) HLA-DRB1*12阳性组IL-4平均表达水平为(5.947±4.530)ng/ml,阴性组平均表达水平为(5.132±5.800) ng/ml,两组间差异无统计学意义(P=0.423).结论 HLA-DRB1*12基因可能是促进广西人群乙肝疫苗免疫应答的相关基因；细胞因子IFN-γ及IL-4的表达水平可能影响机体乙肝疫苗的免疫效果.
目的 探討人群HLA-DRB1*12基因及相應位點下IL-4及IFN-γ對乙肝疫苗免疫應答的影響.方法 選取74名對乙肝疫苗無、弱應答的廣西籍健康大學生及條件相近的64名中、彊應答者,採用PCR-SSP技術檢測受試者的HLA-DRB1* 12等位基因,採用ELISA法檢測血清中IFN-γ水平及IL-4因子水平.結果 (1)無、弱應答組的HLA-DRB1*12等位基因錶達頻率為10.8％,低于中、彊應答組的32.8％ (P=0.002)；(2)無、弱應答組的IFN-γ平均錶達水平為(7.21±7.92) ng/ml,顯著低于中、彊應答組(16.36±11.00) ng/ml( P=0.000)；(3)無、弱應答組中的IL-4平均錶達水平為(3.18±4.45) ng/ml,顯著低于中、彊應答組(7.76±5.71) ng/ml(P=0.000)；(4) HLA-DRB1* 12暘性組IFN-γ平均錶達水平為(13.18±11.24) ng/ml,陰性組平均錶達水平為(11.00±10.29) ng/ml,兩組間差異無統計學意義(P=0.349)；(5) HLA-DRB1*12暘性組IL-4平均錶達水平為(5.947±4.530)ng/ml,陰性組平均錶達水平為(5.132±5.800) ng/ml,兩組間差異無統計學意義(P=0.423).結論 HLA-DRB1*12基因可能是促進廣西人群乙肝疫苗免疫應答的相關基因；細胞因子IFN-γ及IL-4的錶達水平可能影響機體乙肝疫苗的免疫效果.
목적 탐토인군HLA-DRB1*12기인급상응위점하IL-4급IFN-γ대을간역묘면역응답적영향.방법 선취74명대을간역묘무、약응답적엄서적건강대학생급조건상근적64명중、강응답자,채용PCR-SSP기술검측수시자적HLA-DRB1* 12등위기인,채용ELISA법검측혈청중IFN-γ수평급IL-4인자수평.결과 (1)무、약응답조적HLA-DRB1*12등위기인표체빈솔위10.8％,저우중、강응답조적32.8％ (P=0.002)；(2)무、약응답조적IFN-γ평균표체수평위(7.21±7.92) ng/ml,현저저우중、강응답조(16.36±11.00) ng/ml( P=0.000)；(3)무、약응답조중적IL-4평균표체수평위(3.18±4.45) ng/ml,현저저우중、강응답조(7.76±5.71) ng/ml(P=0.000)；(4) HLA-DRB1* 12양성조IFN-γ평균표체수평위(13.18±11.24) ng/ml,음성조평균표체수평위(11.00±10.29) ng/ml,량조간차이무통계학의의(P=0.349)；(5) HLA-DRB1*12양성조IL-4평균표체수평위(5.947±4.530)ng/ml,음성조평균표체수평위(5.132±5.800) ng/ml,량조간차이무통계학의의(P=0.423).결론 HLA-DRB1*12기인가능시촉진엄서인군을간역묘면역응답적상관기인；세포인자IFN-γ급IL-4적표체수평가능영향궤체을간역묘적면역효과.
Objective To elucidate the association of immune response to hepatitis B vaccination with HLA-DRB1 * 12 allele as well as the level of IL-4 and IFN-γ.Methods Seventy-four healthy college students from Guangxi province who had non- or hypor -response to recombinant hepatitis B vaccination and 64 medium- or hyper-responders with the conditions of similar were selected randomly and involved in this study.HLA-DRB1 * 12 was detected by PCR-SSP,the level of IFN-γ and IL-4 cytokines were examined by ELISA.Results (1)The allelic frequencies of HLA-DRB1 * 12 was lower in the non- or hypor-responders than that in the medium- or hyper-responders ( 10.8％ vs 32.8％,P=0.002) ; (2)The expression level of IFN-γ in the non- or hypor-responders ( 7.21±7.92 ) ng/ml was much less than that of the medium- or hyper- responders ( 16.36± 11.00) ng/ml ( P=0.000).(3) The expression level of IL-4 in the non- or hyporresponders (3.18±4.45) ng/ml was much less than that of the medium- or hyper- responders (7.76±5.71 ) ng/ml(P=0.000).(4)No significant differences was seen between the expression level of IFN-γ in the HLA-DRB1 * 12 positive ( 13.18± 11.24) ng/ml and the negative ( 11.00± 10.29 ) ng/ml ( P =0.349 ).(5)No significant differences was seen between the expression level of IL-4 in the HLA-DRB1 * 12 positive (5.947±4.530) ng/ml and the negative (5.132±5.800) ng/ml (P=0.423).Conclusion HLA-DRB1 * 12 might be the allele enhanced immune response to hepatitis B vaccination.The expression levels of IFN-γand IL-4 correlating to Thl/Th2 cells might affect on the immune response to hepatitis B vaccination.
