中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
6期
549-554
,共6页
王悦%宋诗铎%祁伟%刘德梦%王玉宝%王哲%郭文学
王悅%宋詩鐸%祁偉%劉德夢%王玉寶%王哲%郭文學
왕열%송시탁%기위%류덕몽%왕옥보%왕철%곽문학
鲍曼不动杆菌%多重耐药性%外排泵
鮑曼不動桿菌%多重耐藥性%外排泵
포만불동간균%다중내약성%외배빙
Acinetobacter baumannii%Multi-drug resistance%Efflux pump
目的 探讨鲍曼不动杆菌临床株外排泵AdeABC、AdeIJK、AdeFGH、AbeM、AbeS、CraA、MdtL的表达与耐药的关系.方法 收集多重耐药鲍曼不动杆菌临床株32株和敏感株10株,PCR扩增泵基因；选取主要克隆型的21株多重耐药株和10株敏感株,实时荧光定量RT-PCR方法检测泵基因adeB、adeJ、adeG、abeM、abeS、craA、mdtL的mRNA相对表达水平,PCR扩增泵调控基因adeRS、adeL并测序分析.结果 32株多重耐药鲍曼不动杆菌临床株中携带泵结构基因片段adeB100％、adeJ 100％、adeG 100％、abeM 96.88％、abeS 100％、craA 100％、mdtL 93.75％,10株敏感株均存在7种泵结构基因片段；主要克隆型的21株多重耐药株和10株敏感株adeB、abeM、mdtL的mRNA相对表达水平的差异有统计学意义( P＜0.001,P=0.001,P=0.013),选取多重耐药株AbR3和AbR11检测外排泵AdeABC调控基因adeRS序列出现氨基酸替代及缺失,而外排泵AdeFGH调控基因adeL序列无基因突变.结论 鲍曼不动杆菌临床株外排泵AdeABC、AbeM、MdtL的表达可能与耐药性有关.
目的 探討鮑曼不動桿菌臨床株外排泵AdeABC、AdeIJK、AdeFGH、AbeM、AbeS、CraA、MdtL的錶達與耐藥的關繫.方法 收集多重耐藥鮑曼不動桿菌臨床株32株和敏感株10株,PCR擴增泵基因；選取主要剋隆型的21株多重耐藥株和10株敏感株,實時熒光定量RT-PCR方法檢測泵基因adeB、adeJ、adeG、abeM、abeS、craA、mdtL的mRNA相對錶達水平,PCR擴增泵調控基因adeRS、adeL併測序分析.結果 32株多重耐藥鮑曼不動桿菌臨床株中攜帶泵結構基因片段adeB100％、adeJ 100％、adeG 100％、abeM 96.88％、abeS 100％、craA 100％、mdtL 93.75％,10株敏感株均存在7種泵結構基因片段；主要剋隆型的21株多重耐藥株和10株敏感株adeB、abeM、mdtL的mRNA相對錶達水平的差異有統計學意義( P＜0.001,P=0.001,P=0.013),選取多重耐藥株AbR3和AbR11檢測外排泵AdeABC調控基因adeRS序列齣現氨基痠替代及缺失,而外排泵AdeFGH調控基因adeL序列無基因突變.結論 鮑曼不動桿菌臨床株外排泵AdeABC、AbeM、MdtL的錶達可能與耐藥性有關.
목적 탐토포만불동간균림상주외배빙AdeABC、AdeIJK、AdeFGH、AbeM、AbeS、CraA、MdtL적표체여내약적관계.방법 수집다중내약포만불동간균림상주32주화민감주10주,PCR확증빙기인；선취주요극륭형적21주다중내약주화10주민감주,실시형광정량RT-PCR방법검측빙기인adeB、adeJ、adeG、abeM、abeS、craA、mdtL적mRNA상대표체수평,PCR확증빙조공기인adeRS、adeL병측서분석.결과 32주다중내약포만불동간균림상주중휴대빙결구기인편단adeB100％、adeJ 100％、adeG 100％、abeM 96.88％、abeS 100％、craA 100％、mdtL 93.75％,10주민감주균존재7충빙결구기인편단；주요극륭형적21주다중내약주화10주민감주adeB、abeM、mdtL적mRNA상대표체수평적차이유통계학의의( P＜0.001,P=0.001,P=0.013),선취다중내약주AbR3화AbR11검측외배빙AdeABC조공기인adeRS서렬출현안기산체대급결실,이외배빙AdeFGH조공기인adeL서렬무기인돌변.결론 포만불동간균림상주외배빙AdeABC、AbeM、MdtL적표체가능여내약성유관.
Objective To study the expression of active efflux pump AdeABC,AdeIJK,AdeFGH,AbeM,AbeS,CraA,MdtL in clinical Acinetobacter baumannii isolates and whether the efflux pumps confers resistance to antibiotics.Methods Thirty-two multi-drug resistant Acinetobacter baumannii islates and 10 sensitive isolates were collected.Genes of the exporter protein were amplified by PCR.The expression of adeB,adeJ,adeG,abeM,abeS,craA,mdtL were examined by real-time fluorescence quantitative RT-PCR.The controlling genes adeRS and adeL were amplified by PCR and sequenced.Results The positivity rates of adeB,adeJ,adeG,abeM,abeS,craA,mdtL were 100％,100％,100％,96.88％,100％,100％ and 93.75％ respectively in 32 multi-drug resistant Acinetobacter baumannii isolates,and all 100％ in 10 sensitive isolates.The difference of expression of adeB,abeM and mdtL were significant( P＜0.001,P =0.001,P=0.013) between 21 multi-drug resistant isolates of C clone and 10 sensitive isolates.The mutations of adeRS existed in 2 multi-drug resistant isolates,no point mutation of adeL.Conclusion The expression of AdeABC,AbeM and MdtL may involved in the resistant mechanisms of the clinical Acinetobacter baumannii islates.
