中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
28期
1970-1973
,共4页
谢辉%陈洪德%黄慧聪%杨亦荣%吴建波%何秋香%朱启建%陈建欧%李澄棣%夏鹏%沈龙捷
謝輝%陳洪德%黃慧聰%楊亦榮%吳建波%何鞦香%硃啟建%陳建歐%李澄棣%夏鵬%瀋龍捷
사휘%진홍덕%황혜총%양역영%오건파%하추향%주계건%진건구%리징체%하붕%침룡첩
前列腺炎%基因,rRNA%免疫球蛋白A%细胞因子类%细菌
前列腺炎%基因,rRNA%免疫毬蛋白A%細胞因子類%細菌
전렬선염%기인,rRNA%면역구단백A%세포인자류%세균
Prostatitis%Genes,rRNA%Immuneglobulin A%Cytokines%Bacteria
目的 探讨细菌在慢性前列腺炎(CP)中的致病作用.方法 前列腺标本取自2002-2008年192例猝死于非前列腺疾病的器官捐献者,年龄20～38岁.取周围带组织并分两块,一块前列腺组织行病理检查及白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、免疫球蛋白A(IgA)的免疫组化分析;另一块行细菌16S rRNA基因(16S rDNA)PCR分析.结果 33.3%(64/192)的前列腺组织病理呈CP改变.细菌16S rDNA总阳性率为19.8%(38/192),而在CP标本中16S rDNA阳性率为50.0%(32/64),非CP标本中16S rDNA阳性率为4.6%(6/128),CP组16S rDNA阳性率高于非CP组(x2=55.185,P<0.001).IL-1β、TNF-α和IgA的表达在CP组中明显高于非CP标本(P<0.01),且三者表达呈正相关(P<0.01);在64例CP组织标本中,16S rDNA阳性者IL-1β、TNF-α和IgA的表达明显高于16S rDNA阴性者(P<0.01).结论 前列腺组织中细菌16S rDNA、细胞因子和免疫球蛋白A的表达增加和前列腺组织病理炎症改变相关,提示细菌感染可能是CP的重要病因.
目的 探討細菌在慢性前列腺炎(CP)中的緻病作用.方法 前列腺標本取自2002-2008年192例猝死于非前列腺疾病的器官捐獻者,年齡20～38歲.取週圍帶組織併分兩塊,一塊前列腺組織行病理檢查及白細胞介素1β(IL-1β)、腫瘤壞死因子α(TNF-α)、免疫毬蛋白A(IgA)的免疫組化分析;另一塊行細菌16S rRNA基因(16S rDNA)PCR分析.結果 33.3%(64/192)的前列腺組織病理呈CP改變.細菌16S rDNA總暘性率為19.8%(38/192),而在CP標本中16S rDNA暘性率為50.0%(32/64),非CP標本中16S rDNA暘性率為4.6%(6/128),CP組16S rDNA暘性率高于非CP組(x2=55.185,P<0.001).IL-1β、TNF-α和IgA的錶達在CP組中明顯高于非CP標本(P<0.01),且三者錶達呈正相關(P<0.01);在64例CP組織標本中,16S rDNA暘性者IL-1β、TNF-α和IgA的錶達明顯高于16S rDNA陰性者(P<0.01).結論 前列腺組織中細菌16S rDNA、細胞因子和免疫毬蛋白A的錶達增加和前列腺組織病理炎癥改變相關,提示細菌感染可能是CP的重要病因.
목적 탐토세균재만성전렬선염(CP)중적치병작용.방법 전렬선표본취자2002-2008년192례졸사우비전렬선질병적기관연헌자,년령20～38세.취주위대조직병분량괴,일괴전렬선조직행병리검사급백세포개소1β(IL-1β)、종류배사인자α(TNF-α)、면역구단백A(IgA)적면역조화분석;령일괴행세균16S rRNA기인(16S rDNA)PCR분석.결과 33.3%(64/192)적전렬선조직병리정CP개변.세균16S rDNA총양성솔위19.8%(38/192),이재CP표본중16S rDNA양성솔위50.0%(32/64),비CP표본중16S rDNA양성솔위4.6%(6/128),CP조16S rDNA양성솔고우비CP조(x2=55.185,P<0.001).IL-1β、TNF-α화IgA적표체재CP조중명현고우비CP표본(P<0.01),차삼자표체정정상관(P<0.01);재64례CP조직표본중,16S rDNA양성자IL-1β、TNF-α화IgA적표체명현고우16S rDNA음성자(P<0.01).결론 전렬선조직중세균16S rDNA、세포인자화면역구단백A적표체증가화전렬선조직병리염증개변상관,제시세균감염가능시CP적중요병인.
Objective To investigate the role of bacteria in the etiology of chronic prostatitis.Methods Complete prostate specimens were obtained at autopsy from 192 organ donors (aged 20 - 38 years old) during 2002 to 2008 who died of non-prostatic diseases.One tissue taken from the peripheral prostatic zone according to McNeal was divided into two pieces.One piece of tissue was taken for routine pathological examinations and immunohistochemical studies of interleukin (IL) -1β, tumor necrosis factor-α (TNF-α)and IgA.Another one was taken for PCR assay to detect the bacterial 16S rRNA genes ( 16S rDNA ).Results Of 192 prostate specimens, 64 (33.3%) had pathological changes of chronic prostatitis and 38 ( 19.8% ) specimens was positive for bacterial 16S rDNA.Positive rates of 16S rDNA in chronic prostatitis and non-prostatitis specimens were 50.0% (32/64) and 4.6% (6/128) respectively ( X2 = 55.185, P <0.001 ).Expressions of IL-1 β, TNF-α and IgA in specimens of chronic prostatitis were significantly higher than those in non-prostatitis specimens ( P < 0.001 ).A positive correlation could be found among three immunohistochemical indicators (P < 0.01 ).In 64 specimens with chronic prostatitis, a significant expression of IL-1β, TNF-α and IgA was more often demonstrated in 16S rDNA positive group than in 16S rDNA negative group (P <0.001 ).Conclusions The up-regulations of bacterial 16S rDNA, cytokines and immunoglobulin A are involved in inflammatory response of chronic prostatitis.Bacterial infection may be an important cause of chronic prostatitis.
