CAJ | 학술논문

目的了解本实验室2004-2006年分离的7株B亚属腺病毒(adenovirus,Ad)的纤维基因特征,并与日本、韩国腺病毒分离株的纤维基因进行比较.方法 2004-2006年间,本实验室从上呼吸道感染儿童散发或暴发病例中分离到7株腺病毒毒株,首先对六邻体蛋白基因扩增和序列分析进行基因分型,随后用PCR扩增纤维蛋白基因,进行序列测定,构建亲缘性关系进化树,并对其基因特征进行分析.结果 7株腺病毒分离株均属于B亚属,其中有3株为Ad3型,所测定的纤维蛋白基因的核苷酸同源性为98.9%～100%,与2003年中国广州分离株DQ099432和2003年韩国分离株AY224416的同源性为100%;3株为Ad7型,纤维蛋白基因核苷酸同源性为97.6%～100%,与2006年日本分离株AB243119的同源性为97.4%,与2000年韩国分离株AY921620的同源性为100%;1株为Ad11型,与GenBank序列号为L08232的1993年瑞典分离株纤维蛋白基因同源性为100%.结论 2004-2006年,北京地区上呼吸道感染散发病例,Ad3和Ad7纤维基因核苷酸变异不大,同时证实本研究用纤维基因片段,与六邻体基因分型具有相同的结果.
목적 료해본실험실2004-2006년분리적7주B아속선병독(adenovirus,Ad)적섬유기인특정,병여일본、한국선병독분리주적섬유기인진행비교.방법 2004-2006년간,본실험실종상호흡도감염인동산발혹폭발병례중분리도7주선병독독주,수선대륙린체단백기인확증화서렬분석진행기인분형,수후용PCR확증섬유단백기인,진행서렬측정,구건친연성관계진화수,병대기기인특정진행분석.결과 7주선병독분리주균속우B아속,기중유3주위Ad3형,소측정적섬유단백기인적핵감산동원성위98.9%～100%,여2003년중국엄주분리주DQ099432화2003년한국분리주AY224416적동원성위100%;3주위Ad7형,섬유단백기인핵감산동원성위97.6%～100%,여2006년일본분리주AB243119적동원성위97.4%,여2000년한국분리주AY921620적동원성위100%;1주위Ad11형,여GenBank서렬호위L08232적1993년서전분리주섬유단백기인동원성위100%.결론 2004-2006년,북경지구상호흡도감염산발병례,Ad3화Ad7섬유기인핵감산변이불대,동시증실본연구용섬유기인편단,여륙린체기인분형구유상동적결과.
Objective To analyze the genotypes of adenovirus(Ad)strains isolated from young children with acute respiratory infection syndrome during 2004-2006.Methods Seven human adenovirus strains isolated during 2004 and 2006 were detected with tissue culture,PCR with adenovims specific-primers and sequencing for hexon and fiber genes.Results The results showed that all 7 adenovirus strain belonged to adenovirus genera B.3 of the 7 isolates were Ad3,BJ060605-1,BJ060316-and BJ040228 had high homology of nucleic acid.98.9%-100%.Three of 7 were Ad7,the homology of nucleic acid of BJ050520.BJ060421-4 and BJ040226 were 97.6%-100%.Strain SX060418-1 was 100% homologic with Ad11 strain L08232(GenBank).Conclusion Little variation of fiber gene was found for 2004 to 2006 isolares and the typing with fiber sequencing is similar with that of hexon gene of adenovirus isolates.