CAJ | 학술논문

研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性(SRAP).结果表明,SARP技术可以揭示花生栽培种的遗传差异.在所试验的51对SRAP 引物中,2对只获得了单态性条带,其余49对总共产生了1087 条带,其中 503 条(46.27%) 为多态性带, 每对引物组合平均获得22.18条总带、10.26 条多态性带. 在这49对引物组合中,总带数和多态性条带的数目分别为7～63 和 2～32 条. 10个花生栽培种间的简单匹配相似系数为0.7712～0.9250不等,根据SRAP指纹图谱进行聚类分析可将这10个品种分为 4 类.另外一项采用作图亲本24-3和B4的研究中, 40对SRAP引物共计获得了160条多态性带.本研究为花生品种鉴定和遗传研究提供了新的DNA分子标记技术手段.
연구료분속3개시장형적10개중국화생재배충적서렬상관확증다태성(SRAP).결과표명,SARP기술가이게시화생재배충적유전차이.재소시험적51대SRAP 인물중,2대지획득료단태성조대,기여49대총공산생료1087 조대,기중 503 조(46.27%) 위다태성대, 매대인물조합평균획득22.18조총대、10.26 조다태성대. 재저49대인물조합중,총대수화다태성조대적수목분별위7～63 화 2～32 조. 10개화생재배충간적간단필배상사계수위0.7712～0.9250불등,근거SRAP지문도보진행취류분석가장저10개품충분위 4 류.령외일항채용작도친본24-3화B4적연구중, 40대SRAP인물공계획득료160조다태성대.본연구위화생품충감정화유전연구제공료신적DNA분자표기기술수단.
SRAP assay was conducted using 10 peanut cultivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. Of the primer pairs tested, 2 produced monomorphic bands only, and 49 totally produced 1087 bands, among which 503 (46.27%) were polymorphic, with an average of 10.26 polymorphic bands and 22.18 bands per primer combination. The total number of bands and polymorphic bands in the 49 primer combinations ranged from 7～63 and 2～32 respectively. The simple matching coefficient of the peanut varieties ranged from 0.7712 to 0.9250. The varieties may be grouped into 4 categories based on cluster analysis of the SRAP fingerprinting data. In a separate study on SARP using 40 primer pairs, 160 polymorphic bands were detected in 24-3 and B4,the parental genotypes for mapping. This study provided an additional marker system for peanut variety identification and genetic research.