CAJ | 학술논문

目的:研究慢性高眼压过程中大鼠视网膜Caspase-9表达的变化及应用AG对其表达的影响,探讨AG可能存在的对慢性高眼压视网膜的保护作用.方法:应用免疫组化、RT-PCR和Western blot的方法观察应用AG及未应用AG的慢性高眼压大鼠视网膜在慢性高眼压后不同时间点的形态学变化及Caspase-9表达的变化.结果:与正常对照组相比,随着高眼压时间的延长视网膜逐渐出现可察觉的形态学变化,于高眼压的第21d视网膜变薄,节细胞数量减少;在此过程中,Caspase-9表达增多,与形态学变化相一致.而应用AG者其形态学变化和Caspase-9表达变化较小.结论:凋亡相关基因Caspase-9在慢性高眼压视网膜损伤过程中发挥了作用,AG通过下调其表达对视网膜起保护作用.
목적:연구만성고안압과정중대서시망막Caspase-9표체적변화급응용AG대기표체적영향,탐토AG가능존재적대만성고안압시망막적보호작용.방법:응용면역조화、RT-PCR화Western blot적방법관찰응용AG급미응용AG적만성고안압대서시망막재만성고안압후불동시간점적형태학변화급Caspase-9표체적변화.결과:여정상대조조상비,수착고안압시간적연장시망막축점출현가찰각적형태학변화,우고안압적제21d시망막변박,절세포수량감소;재차과정중,Caspase-9표체증다,여형태학변화상일치.이응용AG자기형태학변화화Caspase-9표체변화교소.결론:조망상관기인Caspase-9재만성고안압시망막손상과정중발휘료작용,AG통과하조기표체대시망막기보호작용.
AIM: To study caspase-9 expression on rat retina in the process of chronic elevation of IOP and the changes with the application of amino guanidine (AG), thus to investigate potential protective function of AG to rat retina with chronic elevation of IOP.METHODS: Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and expression of caspase-9 at different time points of rat with chronic IOP elevation, both affected or not affected by the application of AG.RESULTS: Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21st day of chronic IOP elevation, retinas became thinner and the quantity of retinal ganglion cells (RGCs) decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9.CONCLUSION: Apoptosis-related gene caspase-9 played a part in the process of chronic IOP elevation; AG protects retina by down-regulating expression of caspase-9.