CAJ | 학술논문

目的研究难治性癫(癎)(RE)患儿多药耐药基因(MDR1)的表达及其临床意义.方法提取难治性癫(癎)患儿(n=30)、非难治性癫(癎)患儿(n=30)和正常健康儿童(n=30)外周血标本,用荧光定量PCR方法分析比较MDR1 mRNA在各组的表达.同时观察MDR1 mRNA水平与癫(癎)发作频率和应用抗癫(癎)药物(AEDs)的种类与数量关系.结果 MDR1 mRNA在难治性癫(癎)组表达明显高于非难治性癫(癎)组及正常对照组(P均<0.01);在发作次数频繁患儿明显高于发作次数较少患儿(P<0.01);在使用4种AEDs患儿明显高于使用2种及3种AEDs息儿(P<0.05).结论血中高表达的MDR1参与了难治性癫(癎)的耐药机制,可作为判断难治性癫(癎)的客观指标.
목적 연구난치성전(간)(RE)환인다약내약기인(MDR1)적표체급기림상의의.방법 제취난치성전(간)환인(n=30)、비난치성전(간)환인(n=30)화정상건강인동(n=30)외주혈표본,용형광정량PCR방법분석비교MDR1 mRNA재각조적표체.동시관찰MDR1 mRNA수평여전(간)발작빈솔화응용항전(간)약물(AEDs)적충류여수량관계.결과 MDR1 mRNA재난치성전(간)조표체명현고우비난치성전(간)조급정상대조조(P균<0.01);재발작차수빈번환인명현고우발작차수교소환인(P<0.01);재사용4충AEDs환인명현고우사용2충급3충AEDs식인(P<0.05).결론 혈중고표체적MDR1삼여료난치성전(간)적내약궤제,가작위판단난치성전(간)적객관지표.
Objective To study the expression and significance of multidrug resistance gene (MDR1) in children with refractory epilepsy (RE). Methods Children with RE (n = 30), non-RE (n = 30) and healthy children (n=30) were collected. The expression of MDR1-mRNA in peripheral blood was analyzed by fluorescence quantitative PCR. The relationship of MDRI-mRNA with epileptic frequency and numbers of antiepileptic drugs (AEDs) were observed. Results The expression of MDR1 in RE group obviously increased when compared with that of non-RE group and healthy group (P < 0.01, P < 0.01) ; MDR1 expression was more among patients with high frequent epilepsy than patients with low frequent epilepsy (P < 0.01) ; more in patients administered with four kinds of AEDs than those with two or three kinds of AEDs (P < 0.01). Concinsions MDR1 overexpression in blood of children with RE may be linked to drug-resistant mechanism of RE. It might be used as a clinical indicator of RE.