中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2009年
6期
518-522
,共5页
曲马多%O-去甲基曲马多%反相高效液相色谱
麯馬多%O-去甲基麯馬多%反相高效液相色譜
곡마다%O-거갑기곡마다%반상고효액상색보
tramadol%O - demethyltramadol%RP - HPLC
目的 建立血浆和尿液中曲马多(镇痛药)及其代谢产物O-去甲基曲马多的反相高效液相色谱分析方法.方法 用zorbax SB-C_(18)色谱柱,0.05 mol·L~(-1)KH_2PO_4(用磷酸调节pH至4.0)-乙腈(90:10)为流动相,流量1.0 mL·min~(-1);荧光检测波长E_x=216 nm,E_m=308 nm.结果 血浆中,曲马多、O-去甲基曲马多的线性范围分别为12.5～800.0、5～320 ng·mL~(-1),低、中、高3种浓度绝对回收率均大于86%,相对回收率为93%～105%,日内和日间RSD分别小于9%和8%.尿液中,曲马多、O-去甲基曲马多线性范围分别为12.5～4 000、5～1 280 ng·mL~(-1),低、中、高3种浓度绝对回收率均大于80%,相对回收率为91%～107%,日内和日间RSD均小于9%.在血浆和尿液中均稳定,差异均在±10%以内.结论 本方法准确可靠、专一性好、操作简便.
目的 建立血漿和尿液中麯馬多(鎮痛藥)及其代謝產物O-去甲基麯馬多的反相高效液相色譜分析方法.方法 用zorbax SB-C_(18)色譜柱,0.05 mol·L~(-1)KH_2PO_4(用燐痠調節pH至4.0)-乙腈(90:10)為流動相,流量1.0 mL·min~(-1);熒光檢測波長E_x=216 nm,E_m=308 nm.結果 血漿中,麯馬多、O-去甲基麯馬多的線性範圍分彆為12.5～800.0、5～320 ng·mL~(-1),低、中、高3種濃度絕對迴收率均大于86%,相對迴收率為93%～105%,日內和日間RSD分彆小于9%和8%.尿液中,麯馬多、O-去甲基麯馬多線性範圍分彆為12.5～4 000、5～1 280 ng·mL~(-1),低、中、高3種濃度絕對迴收率均大于80%,相對迴收率為91%～107%,日內和日間RSD均小于9%.在血漿和尿液中均穩定,差異均在±10%以內.結論 本方法準確可靠、專一性好、操作簡便.
목적 건립혈장화뇨액중곡마다(진통약)급기대사산물O-거갑기곡마다적반상고효액상색보분석방법.방법 용zorbax SB-C_(18)색보주,0.05 mol·L~(-1)KH_2PO_4(용린산조절pH지4.0)-을정(90:10)위류동상,류량1.0 mL·min~(-1);형광검측파장E_x=216 nm,E_m=308 nm.결과 혈장중,곡마다、O-거갑기곡마다적선성범위분별위12.5～800.0、5～320 ng·mL~(-1),저、중、고3충농도절대회수솔균대우86%,상대회수솔위93%～105%,일내화일간RSD분별소우9%화8%.뇨액중,곡마다、O-거갑기곡마다선성범위분별위12.5～4 000、5～1 280 ng·mL~(-1),저、중、고3충농도절대회수솔균대우80%,상대회수솔위91%～107%,일내화일간RSD균소우9%.재혈장화뇨액중균은정,차이균재±10%이내.결론 본방법준학가고、전일성호、조작간편.
Objective To establish analytical method for the determination of tramadol and its metabolite O - demethyltramadol in human plasma and urine by RP - HPLC. Methods Separation was carried out ons a zorbax RP - select B column and the mobile phase was a mixture of 0. 05 mol · L~(-1) potassium dihydrogen phosphate ( adjusted to pH 4. 0 with phosphoric acid)-acetonitrile (90: 10). The flow rate was 1 mL · min~(-1). Fluormetric detection was performed at excitation and emission wavelengths of 216 run and 308 nm, respectively. Results In plasma, the calibration curve was linear within 12. 5 - 800. 0 ng · mL~(-1) for tramadol and 5 -320 ng · mL~(-1) for O - demethyltramadol. The absolute recoveries were all higher than 86% , and the relative recoveries were from 93% to 105%. The RSD of the within - day and between - day variations were lower than 9% and 8% , respectively. In urine, the calibration curve was linear within 12. 5 -4 000 ng · mL~(-1) for tramadol and 5 -1 280 ng · mL~(-1) for O -demethyltramadol. The absolute recoveries were all higher than 80% , and the relative recoveries were from 91% to 107%. The RSD of the within-day and between-day variations were lower than 9%. For stability test in plasma and urine, the difference of the results was within 10%. Conclusion This RP - HPLC method is simple, sensitive, accurate and specific.
