CAJ | 학술논문

[目的]鉴定转基因鱼腥草中外源抗菌肽的表达,分析抗茎腐病的能力.[方法]通过SDS-PAG电泳和Western blot方法检测转基因鱼腥草植株中外源抗菌肽的表达,接种不同浓度的立枯丝核菌孢子,比较转基因植株与非转基因植株的染病情况.[结果]外源抗菌肽能在转基因鱼腥草中表达;立枯丝核菌的最佳侵染孢子浓度为3×105/mL,观察时间以3 d为宜,转基因鱼腥草较非转基因鱼腥草的抗茎腐病能力增强.[结论]外源抗菌肽的表达能增强转基因鱼腥草植株的抗茎腐病能力.
[목적]감정전기인어성초중외원항균태적표체,분석항경부병적능력.[방법]통과SDS-PAG전영화Western blot방법검측전기인어성초식주중외원항균태적표체,접충불동농도적립고사핵균포자,비교전기인식주여비전기인식주적염병정황.[결과]외원항균태능재전기인어성초중표체;립고사핵균적최가침염포자농도위3×105/mL,관찰시간이3 d위의,전기인어성초교비전기인어성초적항경부병능력증강.[결론]외원항균태적표체능증강전기인어성초식주적항경부병능력.
[Objective] This study was to identify the expression of exogenous antimicrobial peptide in transgenic Houttuynia cordata Thunb.plants, and analyze their resistance to stem rot disease. [Methods] SDS-PAGE and Western blot analysis were employed to detect expression of exogenous antimicrobial peptide in transgenic H. cordata plants. Both wild type and transgenic H. cordata plants were inoculated with different concentrations of Rhizoctonia solani spores for detecting their resistance. [Results] The exogenous antimicrobial peptide was detected at translation level. The optimal parameters for detecting the resistance of transgenic H. cordata plants to R. solani was inoculation of spores at a concentration of 3 x105 ind./ml and cultured for three days. The results showed that resistance of transgenic H. cordata plants to R. solani was enhanced in comparison with CKs. [Conclusion] Expression of exogenous antimicrobial peptide can enhance the resistance of transgenic H. cordata plants to stem rot disease.