地方性砷中毒对机体氧化应激和免疫功能的远期影响
지방성신중독대궤체양화응격화면역공능적원기영향
Long-term effect of endemic arsenism on oxidative stress and immune function
  • 万方数据
    中国地方病学杂志 중국지방병학잡지
    CHINESE JOURNAL OF ENDEMIOLOGY
    2011年 1期 16-19 ,共4页

    李军%王三祥%王正辉%程晓天%郭百锁%张向东%贾清珍%乔小艳%吴赵明

    리군%왕삼상%왕정휘%정효천%곽백쇄%장향동%가청진%교소염%오조명

    砷中毒%氧化性应激%免疫%时间 砷中毒%氧化性應激%免疫%時間
    신중독%양화성응격%면역%시간
    Arsenic poisoning%Oxidative stress%Immunity%Time
    目的 观察地方性砷中毒(简称地砷病)对机体氧化应激及免疫功能的远期影响,为地砷病病区居民的预防和治疗提供科学依据.方法 2009年,在改水5年的地砷病病区(山西省山阴县古城镇四里庄村、大营村、古城村)选取轻、中、重度病例作为轻、中、重度病例组,在当地选择健康人群作为内对照组,另选取非病区(合盛堡乡杨庄村)健康人群作为外对照组.采集观察对象血样,检测氧化应激指标[采用黄嘌呤氧化酶法检测血清超氧化物歧化酶(SOD)活力,采用二硫代二硝基苯甲酸分光光度法检测谷胱甘肽过氧化物酶(GSH-Px)活力、采用硫代巴比妥酸分光光度法检测丙二醛(MDA)水平]和免疫功能指标[采用放射免疫(RIA)法检测免疫球蛋白G(IgG),采用比浊法检测血清溶菌酶].结果 本次共调查了252人,外对照组,内对照组,轻、中、重度病例组分别为56、57、49、44、46人.5组血清SOD活力分别为(72.19±11.75)、(66.96±12.02)、(49.79±11.07)、(48.54±10.56)、(47.68±10.68)kU/L,组间比较差异有统计学意义(F=52.42,P＜0.01),外对照组明显高于其他组别(P均＜0.05),内对照组高于3个病例组(P均＜0.05),病例组组间比较差异无统计学意义(P＞0.05);5组血清GSH-Px活力分别为(197.41±38.54)、(195.02±31.93)、(187.26±28.22)、(187.24±25.40)、(186.88±21.84)U/mg,组间比较差异无统计学意义(H=4.21,P＞0.05);5组血清MDA水平分别为(4.51±2.14)、(5.88±2.00)、(6.44±2.83)、(5.89±2.57)、(5.88±2.40)μmol/L,组间比较差异有统计学意义(F=3.36,P＜0.05),外对照组明显低于其他组别(P均＜0.05),其他组间比较差异无统计学意义(P均＞0.05);5组血清IgG水平分别为(11.16±2.08)、(8.15±1.44)、(8.77±2.54)、(9.19±1.97)、(8.44±2.52)g/L,组间比较差异有统计学意义(H=52.92,P＜0.01),外对照组明显高于其他组别(P均＜0.05),其他组间比较差异无统计学意义(P均＞0.05);5组血清溶菌酶水平分别为(13.57±5.16)、(10.05±3.96)、(8.78±3.35)、(8.72±3.76)、(9.38±4.26)mg/L,组间比较差异有统计学意义(H=35.00,P＜0.01),外对照组明显高于其他组别(P均＜0.05),其他组间比较差异无统计学意义(P均＞0.05).结论 地砷病患者饮用低砷水5年后,砷对机体的氧化应激反应及免疫功能影响仍然存在,地砷病病区在加大除砷改水力度的同时,应加强居民身体状况的监测.
    목적 관찰지방성신중독(간칭지신병)대궤체양화응격급면역공능적원기영향,위지신병병구거민적예방화치료제공과학의거.방법 2009년,재개수5년적지신병병구(산서성산음현고성진사리장촌、대영촌、고성촌)선취경、중、중도병례작위경、중、중도병례조,재당지선택건강인군작위내대조조,령선취비병구(합성보향양장촌)건강인군작위외대조조.채집관찰대상혈양,검측양화응격지표[채용황표령양화매법검측혈청초양화물기화매(SOD)활력,채용이류대이초기분갑산분광광도법검측곡광감태과양화물매(GSH-Px)활력、채용류대파비타산분광광도법검측병이철(MDA)수평]화면역공능지표[채용방사면역(RIA)법검측면역구단백G(IgG),채용비탁법검측혈청용균매].결과 본차공조사료252인,외대조조,내대조조,경、중、중도병례조분별위56、57、49、44、46인.5조혈청SOD활력분별위(72.19±11.75)、(66.96±12.02)、(49.79±11.07)、(48.54±10.56)、(47.68±10.68)kU/L,조간비교차이유통계학의의(F=52.42,P＜0.01),외대조조명현고우기타조별(P균＜0.05),내대조조고우3개병례조(P균＜0.05),병례조조간비교차이무통계학의의(P＞0.05);5조혈청GSH-Px활력분별위(197.41±38.54)、(195.02±31.93)、(187.26±28.22)、(187.24±25.40)、(186.88±21.84)U/mg,조간비교차이무통계학의의(H=4.21,P＞0.05);5조혈청MDA수평분별위(4.51±2.14)、(5.88±2.00)、(6.44±2.83)、(5.89±2.57)、(5.88±2.40)μmol/L,조간비교차이유통계학의의(F=3.36,P＜0.05),외대조조명현저우기타조별(P균＜0.05),기타조간비교차이무통계학의의(P균＞0.05);5조혈청IgG수평분별위(11.16±2.08)、(8.15±1.44)、(8.77±2.54)、(9.19±1.97)、(8.44±2.52)g/L,조간비교차이유통계학의의(H=52.92,P＜0.01),외대조조명현고우기타조별(P균＜0.05),기타조간비교차이무통계학의의(P균＞0.05);5조혈청용균매수평분별위(13.57±5.16)、(10.05±3.96)、(8.78±3.35)、(8.72±3.76)、(9.38±4.26)mg/L,조간비교차이유통계학의의(H=35.00,P＜0.01),외대조조명현고우기타조별(P균＜0.05),기타조간비교차이무통계학의의(P균＞0.05).결론 지신병환자음용저신수5년후,신대궤체적양화응격반응급면역공능영향잉연존재,지신병병구재가대제신개수력도적동시,응가강거민신체상황적감측.
    Objective To explore the long-term effect of endemic arsenism on oxidative stress and immune function, and to provide scientific basis for prevention and treatment of the disease in the areas. Methods In 2009, Using cluster sampling and typical investigation, the cross-sectional study was completed. The patient groups and the internal control group were selected in the arsenism areas after 5 years quality improvement of drinking water(Silizhuang village, Daying village and Gucheng village in Shanyin county, Gucheng city, Shanxi province) and they were divided into mild, moderate, severe case and internal control groups, respectively. The external control group was selected in a non-arsenism area(Yangzhuang village in Heshengbu city). The Oxidative stress indicators were determined and analyzed [serum superoxide dismutase (SOD) activity was determined with xanthine oxidase method, glutathione peroxidase(GSH-Px) activity was determined with 2-thio-2-nitrobenzoic acid method, and mmuuity malondisldohyde(MDA) levels was determined with thiobarbituric acid method]. The immune function was determined and analyzed [immunoglobulin G (IgG) was determined with radioimmunoassay method, and serum lysozyme was determined with turbidimetric method]. Results A total of 252 people were surveyed, in which the external control group, the internal control group, mild, moderate and severe patient groups were 56, 57, 49,44 and 46, respectively. Serum SOD activities were (72.19 ± 11.75), (66.96 ± 12.02), (49.79±11.07), (48.54 ±10.56) and (47.68 ± 10.68)kU/L, respectively. The difference of serum SOD activities between the groups was statistically significant(F = 52.42, P ＜ 0.01 ). Serum SOD activities in the external control group were significantly higher than other groups (all P ＜ 0.05). The value in the internal control group was significantly higher than the 3patient groups (all P ＜ 0.05). There were no significant differences between the case groups (P ＞ 0.05). Serum GSH-Px activities of the five groups were (197.41 ± 38.54), (195.02 ± 31.93), (187.26 ± 28.22), (187.24 ± 25.40),(186.88 ± 21.84)U/mg, respectively, and the difference between the groups was not significant(H = 4.21, P ＞0.05). Serum MDA levels of the five groups were (4.51 ± 2.14), (5.88 ± 2.00), (6.44 ± 2.83), (5.89 ± 2.57),(5.88 ± 2.40)μ mol/L, respectively, and the difference between the groups was statistically significant(F = 3.36,P ＜ 0.05). The external control group was significantly lower than other groups(all P ＜ 0.05). No significant difference was observed between other groups(all P ＞ 0.05). Serum IgG levels were(11.16 ± 2.08), (8.15 ± 1.44), (8.77 ±2.54), (9.19 ± 1.97), (8.44 ± 2.52)g/L, respectively, and the difference between the groups was statistically significant(H = 52.92, P ＜ 0.01 ). The external control group was significantly higher than other groups(all P ＜0.05). No significant difference was observed between other groups(all P ＞ 0.05). Serum lysozyme levels were (13.57 ± 5.16), (10.05 ± 3.96), (8.78 ± 3.35), (8.72 ± 3.76), (9.38 ± 4.26)mg/L, respectively, and the difference between the groups was statistically significant (H = 35.00, P ＜ 0.01 ). The external control group was significantly higher than other groups(all P ＜ 0.05). No significant difference was observed between other groups(all P ＞ 0.05). Conclusions The effect of arsenic on the body's oxidative stress response and immune function persists after 5 years of drinking low arsenic water. In addition to intensify arsenic removal from drinking water, it should also strengthen the monitoring of population's health in the diseased areas.
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