中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
4期
383-387
,共5页
陈健平%贾明明%刘宏伟%刘沙%张小青%赵红静%邵一呜%洪坤学
陳健平%賈明明%劉宏偉%劉沙%張小青%趙紅靜%邵一嗚%洪坤學
진건평%가명명%류굉위%류사%장소청%조홍정%소일오%홍곤학
获得性免疫缺陷综合征%HLA-A抗原%HIA-B抗原%HIV-1%病毒载最%CD4淋巴细胞计数
穫得性免疫缺陷綜閤徵%HLA-A抗原%HIA-B抗原%HIV-1%病毒載最%CD4淋巴細胞計數
획득성면역결함종합정%HLA-A항원%HIA-B항원%HIV-1%병독재최%CD4림파세포계수
Acquired immunodeficiency syndrome%HLA-A antigens%HLA-B antigens%HIV-I%Viral load%CD4 lymphocyte count
目的 研究含Bw4表位的HLA-A抗原在HIV-1感染者疾病进展中的作用.方法 应用高分辨率人类白细胞抗原(HLA)分型方法对340例HIV-1感染者进行HLA-A和HLA-B位点的等位基因分型,确定Bw4表位的携带情况,分析HLA-A和HLA-B携带Bw4表位与感染者CD4+4T淋巴细胞计数和血浆病毒载量的关系.结果 与不携带Bw4者(0Bw4)(CD+4T淋巴细胞计数:中位数294个/μ1,病毒载量:中位数6.29×104拷贝/m1)相比,1Bw4-A基因型者和2Bw4-AA基因型者的CD+4T淋巴细胞计数水平(分别为307和308个/μ1)基本相当,而病毒载量(分别为1.53×105和2.68×105拷贝/ml)有增高趋势.与不携带Bw4表位的感染者相比,仅在HLA-B抗原中含Bw4表位的HIV-1感染者的CD+4T淋巴细胞计数(417:个/μ1)显著增高(P=0.013),而病毒载量(2.10×104拷贝/ml)显著降低(P=0.007).结论 含有Bw4表位的HLA-B抗原在HIV-1感染中具有延缓疾病进展的作用,而含有Bw4表位的HLA-A抗原对HIV-1感染者未见保护性作用.
目的 研究含Bw4錶位的HLA-A抗原在HIV-1感染者疾病進展中的作用.方法 應用高分辨率人類白細胞抗原(HLA)分型方法對340例HIV-1感染者進行HLA-A和HLA-B位點的等位基因分型,確定Bw4錶位的攜帶情況,分析HLA-A和HLA-B攜帶Bw4錶位與感染者CD4+4T淋巴細胞計數和血漿病毒載量的關繫.結果 與不攜帶Bw4者(0Bw4)(CD+4T淋巴細胞計數:中位數294箇/μ1,病毒載量:中位數6.29×104拷貝/m1)相比,1Bw4-A基因型者和2Bw4-AA基因型者的CD+4T淋巴細胞計數水平(分彆為307和308箇/μ1)基本相噹,而病毒載量(分彆為1.53×105和2.68×105拷貝/ml)有增高趨勢.與不攜帶Bw4錶位的感染者相比,僅在HLA-B抗原中含Bw4錶位的HIV-1感染者的CD+4T淋巴細胞計數(417:箇/μ1)顯著增高(P=0.013),而病毒載量(2.10×104拷貝/ml)顯著降低(P=0.007).結論 含有Bw4錶位的HLA-B抗原在HIV-1感染中具有延緩疾病進展的作用,而含有Bw4錶位的HLA-A抗原對HIV-1感染者未見保護性作用.
목적 연구함Bw4표위적HLA-A항원재HIV-1감염자질병진전중적작용.방법 응용고분변솔인류백세포항원(HLA)분형방법대340례HIV-1감염자진행HLA-A화HLA-B위점적등위기인분형,학정Bw4표위적휴대정황,분석HLA-A화HLA-B휴대Bw4표위여감염자CD4+4T림파세포계수화혈장병독재량적관계.결과 여불휴대Bw4자(0Bw4)(CD+4T림파세포계수:중위수294개/μ1,병독재량:중위수6.29×104고패/m1)상비,1Bw4-A기인형자화2Bw4-AA기인형자적CD+4T림파세포계수수평(분별위307화308개/μ1)기본상당,이병독재량(분별위1.53×105화2.68×105고패/ml)유증고추세.여불휴대Bw4표위적감염자상비,부재HLA-B항원중함Bw4표위적HIV-1감염자적CD+4T림파세포계수(417:개/μ1)현저증고(P=0.013),이병독재량(2.10×104고패/ml)현저강저(P=0.007).결론 함유Bw4표위적HLA-B항원재HIV-1감염중구유연완질병진전적작용,이함유Bw4표위적HLA-A항원대HIV-1감염자미견보호성작용.
Objective To investigate the impact of HLA-A antigens carrying Bw4 epitopes on disease course of HIV-1 infection.Methods Three hundred and forty subjects chronically infected with HIV-I were recruited and their HLA-A and B alleles were genotyped with sequence-based high resolution typing assay.HLA-Bw genotypes of these HIV-1 infected subjects were determined and their association with CD+4 T cell counts and viral load were analysed.Results When compared with subjects canting no Bw4 epitopes in HLA-A and HLA-B loci (OBw4) (median of CD+4 T cell counts:294/μ1;plasma viral load median 6.29×104 copies/ml),CD+4 T cell counts in subjects with genotypes of 1Bw4-A and 2Bw4-AA were comparable (307 and 308/μ1,respectively),but higher viral load (1.53×105 and 2.68×105 copies/ml,respectively) was observed.In subjects with Bw4 epitopes in HLA-B alleles but no in HLA-A,significantly higher CD+4 T cell counts (417/μ1,P=0.013) and lower viral load (2.10×104 copies/ml,P=0.007) were observed compared with those without Bw4 in HLA-A and HLA-B.Conclusion HLA-B antigens carrying Bw4 epitope were protective in HIV-1 infection by maintaining higher CD+4 T cell counts and lower viral load,but such protective effect was not observed in HLA-A antigens earring Bw4 epitope.
