中国循环杂志
中國循環雜誌
중국순배잡지
CHINESE CIRCULATION JOURNAL
2001年
1期
61-63
,共3页
戴军%高润霖%汤健%宋来凤%魏英杰%宋莉%李永利%唐承君%史瑞文
戴軍%高潤霖%湯健%宋來鳳%魏英傑%宋莉%李永利%唐承君%史瑞文
대군%고윤림%탕건%송래봉%위영걸%송리%리영리%당승군%사서문
支架基因转移平滑肌细胞
支架基因轉移平滑肌細胞
지가기인전이평활기세포
目的：探索蛋白涂层支架携带质粒介导人肝脏诱导型一氧化氮合酶(iNOS)基因转染小型猪冠状动脉可行性。
方法：使用蛋白支架吸附去内毒素纯化质粒，以常规支架置入技术置入小型猪冠状动脉前降支中段。置入后第7天取出前降支置入段，分别提取总核糖核酸(RAN)并进行逆向多聚酶链反应(RT-PCR)，免疫组化染色检测导入人肝脏iNOS蛋白的表达。
结果：小型猪前降支置入支架处显示人iNOS基因信使核糖核酸(mRNA)转录，免疫组化染色显示中膜、内膜人iNOS基因表达人iNOS蛋白的颗粒，以平滑肌细胞最明显。
结论：蛋白涂层支架吸附去内毒素携带人iNOS基因质粒植入小型猪前降支冠状动脉，RT -PCR显示人iNOS基因的mRNA转录，免疫组化显示人iNOS蛋白的表达。
目的：探索蛋白塗層支架攜帶質粒介導人肝髒誘導型一氧化氮閤酶(iNOS)基因轉染小型豬冠狀動脈可行性。
方法：使用蛋白支架吸附去內毒素純化質粒，以常規支架置入技術置入小型豬冠狀動脈前降支中段。置入後第7天取齣前降支置入段，分彆提取總覈糖覈痠(RAN)併進行逆嚮多聚酶鏈反應(RT-PCR)，免疫組化染色檢測導入人肝髒iNOS蛋白的錶達。
結果：小型豬前降支置入支架處顯示人iNOS基因信使覈糖覈痠(mRNA)轉錄，免疫組化染色顯示中膜、內膜人iNOS基因錶達人iNOS蛋白的顆粒，以平滑肌細胞最明顯。
結論：蛋白塗層支架吸附去內毒素攜帶人iNOS基因質粒植入小型豬前降支冠狀動脈，RT -PCR顯示人iNOS基因的mRNA轉錄，免疫組化顯示人iNOS蛋白的錶達。
목적：탐색단백도층지가휴대질립개도인간장유도형일양화담합매(iNOS)기인전염소형저관상동맥가행성。
방법：사용단백지가흡부거내독소순화질립，이상규지가치입기술치입소형저관상동맥전강지중단。치입후제7천취출전강지치입단，분별제취총핵당핵산(RAN)병진행역향다취매련반응(RT-PCR)，면역조화염색검측도입인간장iNOS단백적표체。
결과：소형저전강지치입지가처현시인iNOS기인신사핵당핵산(mRNA)전록，면역조화염색현시중막、내막인iNOS기인표체인iNOS단백적과립，이평활기세포최명현。
결론：단백도층지가흡부거내독소휴대인iNOS기인질립식입소형저전강지관상동맥，RT -PCR현시인iNOS기인적mRNA전록，면역조화현시인iNOS단백적표체。
Objective:To assess the feasibility of plasmid-medicated local transfer of inducible nitric oxide synthase (iNOS) gene to coronary artery using protein-co ated metallic stents in mini-swine medel.
Methods:The metallic stent was coated by cross-linked gelatin and mounted o n 3.0 mm Percutaneous transluminal coronary angioplasty (PTCA) balloon,then End otoxin-free ultrapure Endotoxin-free ultrapure Plasmid pcDNA3hepiNOS under the control of the comegalovirus (CMV) promoter/enhancer was absorbed on the stent. Protein-coated stainless steel stents were used as controls.All stents were imp lanted into the middle segment of left anterior descending artery through 7F lar ge luman gui ding catheter.(The ratio of balloon to vessel diameter was 1.1-1.3∶1).Total RNA of stented segment of coronary artery was extracted,its reverse transeriptio n polymerase chain reaction (RT-PCR) and immunohistochemical staining were perf ormed through routine methods.
Results:At the 7th day after stenting,RT-PCR and immunohistochemical staining confirmed the expression of plasmid pcDNA3hepiNOS mRNA and presence of its protein at gene transered vessels (n＝2) but there was no expression in remote organs.Endothelialization of the vessel was observed in all animals throu gh scanning electromicroscopy.
Conclusions:Local plasmid-mediated human inducible nitric oxide synthase ge ne transfer to coronary artery with protein-coated metallic stents is feasible in mini-swine model.
