CAJ | 학술논문

目的通过观察大鼠脑挫裂伤后脑水肿的磁共振显像(MRI)动态变化,分析中药脑疏宁对脑水肿的干预效果.方法 SD雄性大鼠114只,随机分为脑疏宁组、模型组和假手术组.采用自由落体撞击法建立大鼠脑挫裂伤模型,术后连续动态观察T_2加权像(T_2WI)病灶区信号强度(SI)与体积变化,采用干湿重法测定脑组织含水量.结果外伤后术侧大脑半球见T_2WI高信号改变,1 d时信号强度最高、体积最大,至7 d下降至最低点,与脑组织含水量的变化一致.脑疏宁组各时间点T_2WI信号强度、水肿体积及脑含水量均显著小于模型组(P＜0.05),术后7 d水肿吸收率显著高于模型组(P＜0.005).结论无创性MRI检查能够对大鼠脑外伤及用药后脑水肿程度作出定量分析,是评价药物疗效的可靠手段;中药脑疏宁能够促进大鼠脑挫裂伤后脑水肿的吸收.
목적 통과관찰대서뇌좌렬상후뇌수종적자공진현상(MRI)동태변화,분석중약뇌소저대뇌수종적간예효과.방법 SD웅성대서114지,수궤분위뇌소저조、모형조화가수술조.채용자유락체당격법건립대서뇌좌렬상모형,술후련속동태관찰T_2가권상(T_2WI)병조구신호강도(SI)여체적변화,채용간습중법측정뇌조직함수량.결과 외상후술측대뇌반구견T_2WI고신호개변,1 d시신호강도최고、체적최대,지7 d하강지최저점,여뇌조직함수량적변화일치.뇌소저조각시간점T_2WI신호강도、수종체적급뇌함수량균현저소우모형조(P＜0.05),술후7 d수종흡수솔현저고우모형조(P＜0.005).결론 무창성MRI검사능구대대서뇌외상급용약후뇌수종정도작출정량분석,시평개약물료효적가고수단;중약뇌소저능구촉진대서뇌좌렬상후뇌수종적흡수.
Objective To analyse the effect of Naoshuning on brain edema through observing the dynamic changes of magnetic resonance imaging (MRI) in rats with brain contusion and laceration.Methods All 114 male SD rats were randomly divided into Naoshuning group, model group and sham-operation group. The rat model of brain contusion and laceration was established by free-falling impact according to Feeney's method. After the operation the dynamic changes of MRI signal intensity (SI) and volume in T_2 WI focus were observed consecutively, and the brain water content was determined by using dry-wet method.Results There were changes observed in increased SI of T_2 in the cerebral hemisphere with operation. SI got the highest level and volume on the first day and then decreased gradually and reached the lowest level on the seventh day, which showed a parallel course with brain water content. In Naoshuning group SI in T_2 WI focus, edema volume and brain water content were significantly lower than those in the model group at all time points (P<0.05). The absorption rate of edema in Naoshuning group was significantly higher than that in the model group (P<0.005) seven days after the operation.Conclusion The non-invasive MRI examination can be used to analyse quantitatively the degree of brain trauma and edema in rats after the treatment with Naoshuning, which is reliable for review the curative effect. Naoshuning can improve the absorption of edema after brain contusion and laceration.