CAJ | 학술논문

以粳粳交高产106/长白9号的200个F_(2:3)株系为作图群体,在0.15% Na_2CO_3溶液碱胁迫下,进行了水稻发芽率及其相对碱害率的鉴定评价,并以SSR标记构建的分子连锁图谱为基础,对水稻发芽率及其相对碱害率进行了数量性状基因座(QTL)检测.结果表明,在F_3株系群中水稻发芽率及其相对碱害率均呈单峰接近正态的连续分布.共检测到碱胁迫下与水稻发芽率相关的QTL 7个,对表型变异的贡献率范围为4.05%～12.61%,其中位于第6染色体RM225-RM204区间的qGC-6和位于第9染色体RM219-RM3700区间的qGC-9对表型变异的贡献率分别为12.61%和10.85%.共检测到与水稻发芽率相对碱害率相关的QTL 6个,对表型变异的贡献率为4.82%～28.07%,其中位于第2染色体RM29-RM221区间的qRGC-2、位于第6染色体RM225-RM204区间的qRGC-6-1、位于第9染色体RM219-RM3700区间的qRGC-9和位于第12染色体RM260-RM3226区间的qRGC-12对表型变异的贡献率较大,分别为28.07％、15.35％、15.61％和18.91％,为主效QTL,但其相应的区间距离均较远,需要进一步深入研究.所检测的QTL增效等位基因主要表现为部分显性和超显性.
이갱갱교고산106/장백9호적200개F_(2:3)주계위작도군체,재0.15% Na_2CO_3용액감협박하,진행료수도발아솔급기상대감해솔적감정평개,병이SSR표기구건적분자련쇄도보위기출,대수도발아솔급기상대감해솔진행료수량성상기인좌(QTL)검측.결과표명,재F_3주계군중수도발아솔급기상대감해솔균정단봉접근정태적련속분포.공검측도감협박하여수도발아솔상관적QTL 7개,대표형변이적공헌솔범위위4.05%～12.61%,기중위우제6염색체RM225-RM204구간적qGC-6화위우제9염색체RM219-RM3700구간적qGC-9대표형변이적공헌솔분별위12.61%화10.85%.공검측도여수도발아솔상대감해솔상관적QTL 6개,대표형변이적공헌솔위4.82%～28.07%,기중위우제2염색체RM29-RM221구간적qRGC-2、위우제6염색체RM225-RM204구간적qRGC-6-1、위우제9염색체RM219-RM3700구간적qRGC-9화위우제12염색체RM260-RM3226구간적qRGC-12대표형변이적공헌솔교대,분별위28.07％、15.35％、15.61％화18.91％,위주효QTL,단기상응적구간거리균교원,수요진일보심입연구.소검측적QTL증효등위기인주요표현위부분현성화초현성.
The quantitative trait loci (QTLs) for germination capacity and relative alkali damage rate for germination capacity in rice under alkali stress (0. 15% Na_2CO_3 solution) were identified by using an F_(2:3)population, which included 200 individuals and lines derived from a cross between two japonica cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The germination capacity and its relative alkali damage rate showed a continuous and near normal distribution in F_3 lines. Seven QTLs associated with the germination capacity under alkali stress were detected, which explained 4. 05% to 12. 61 % of the observed phenotypic variances. qGC-6 and qGC-9 were located in RM225-RM204 and RM219-RM3700, respectively, which accounted for 12. 61% and 10. 85% of the observed phenotypic variation, respectively. Six QTLs correlated with relative alkali damage rate for germination capacity under alkali stress were detected. qRGC-2, qRGC-6-1 , qRGC-9 , and qRGC-12 were major QTLs located in RM29-RM221, RM225-RM2O4, RM219-RM3700, and RM260-RM3226, respectively, which explained 28.07%, 15.35%, 15.61% andl8.91% of the observed phenotypic variation, respectively. Given the long distances between the markers further research should be conducted. Most of the alleles were partially dominant or overdominant.