中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2009年
12期
1257-1261
,共5页
陈达%李莹洁%刘艳%朱杰
陳達%李瑩潔%劉豔%硃傑
진체%리형길%류염%주걸
促红细胞生成素%Bcl-2%Bax%脑出血%凋亡%TUNEL%神经保护%损伤
促紅細胞生成素%Bcl-2%Bax%腦齣血%凋亡%TUNEL%神經保護%損傷
촉홍세포생성소%Bcl-2%Bax%뇌출혈%조망%TUNEL%신경보호%손상
Erythropoietin%Bcl-2%Bax%Incerebral hemorrhage( ICH)%Apoptosis%TUNEL%Neuroprotective%Injury
目的 研究促红细胞生成素(EPO)对脑出血神经保护作用的机制.方法 用立体定向技术,将自体小凝血注入大鼠尾状核区制备脑出血模型.110只Wistar雄性大鼠,随机分成四组:正常组、假手术组、ICH对照组、rhEPO治疗组.应用免疫组化及原位细胞凋亡检测脑出血灶周组织Bcl-2、Bax表达及凋亡细胞.统计学方法采用LSD-t检验及Pearson相关分析.结果 ICH对照组及rhEPO治疗组术后6 h血肿周围皮质TUNEL,Bcl-2,Bax阳性细胞明显增加,72 h达到高峰,120 h下降,各时间点与假手术组比较,差异具有统计学意义(P<0.01).rhEPO治疗组TUNEL、Bax阳性细胞数明显少于同时点ICH对照组(P<0.01),但Bcl-2阳性细胞数明显增加(P<0.01).Bax蛋白表达及Bax/Bcl-2均与细胞凋亡呈正相关(P<0.01).结论 凋亡机制参与脑出血后继发性损伤过程,EPO通过上调Bcl-2蛋白表达,下调Bax蛋白表达,对脑出血后神经损伤起保护作用.
目的 研究促紅細胞生成素(EPO)對腦齣血神經保護作用的機製.方法 用立體定嚮技術,將自體小凝血註入大鼠尾狀覈區製備腦齣血模型.110隻Wistar雄性大鼠,隨機分成四組:正常組、假手術組、ICH對照組、rhEPO治療組.應用免疫組化及原位細胞凋亡檢測腦齣血竈週組織Bcl-2、Bax錶達及凋亡細胞.統計學方法採用LSD-t檢驗及Pearson相關分析.結果 ICH對照組及rhEPO治療組術後6 h血腫週圍皮質TUNEL,Bcl-2,Bax暘性細胞明顯增加,72 h達到高峰,120 h下降,各時間點與假手術組比較,差異具有統計學意義(P<0.01).rhEPO治療組TUNEL、Bax暘性細胞數明顯少于同時點ICH對照組(P<0.01),但Bcl-2暘性細胞數明顯增加(P<0.01).Bax蛋白錶達及Bax/Bcl-2均與細胞凋亡呈正相關(P<0.01).結論 凋亡機製參與腦齣血後繼髮性損傷過程,EPO通過上調Bcl-2蛋白錶達,下調Bax蛋白錶達,對腦齣血後神經損傷起保護作用.
목적 연구촉홍세포생성소(EPO)대뇌출혈신경보호작용적궤제.방법 용입체정향기술,장자체소응혈주입대서미상핵구제비뇌출혈모형.110지Wistar웅성대서,수궤분성사조:정상조、가수술조、ICH대조조、rhEPO치료조.응용면역조화급원위세포조망검측뇌출혈조주조직Bcl-2、Bax표체급조망세포.통계학방법채용LSD-t검험급Pearson상관분석.결과 ICH대조조급rhEPO치료조술후6 h혈종주위피질TUNEL,Bcl-2,Bax양성세포명현증가,72 h체도고봉,120 h하강,각시간점여가수술조비교,차이구유통계학의의(P<0.01).rhEPO치료조TUNEL、Bax양성세포수명현소우동시점ICH대조조(P<0.01),단Bcl-2양성세포수명현증가(P<0.01).Bax단백표체급Bax/Bcl-2균여세포조망정정상관(P<0.01).결론 조망궤제삼여뇌출혈후계발성손상과정,EPO통과상조Bcl-2단백표체,하조Bax단백표체,대뇌출혈후신경손상기보호작용.
Objective To study the neuroprotective effects of erythropoietin on intracerebral hemorrhage (ICH). Method The rat models of 1CH were produced by injecting autologous blood into caudate necleus by using stereotatic techique. One hundred ten male wistar rats were randomly divided into 4 groups, namely normal group,sham operation group, 1CU group, and EPO treatment group. The immunohistochemistry and TUNEL were used to detect expressions of Bc 1-2 and Bax,and apoptosis cells. LSD- t and Pearson correlation were used to analyzing data. Results The positive cells of TUNEL Bcl-2 and Bax in ICH group and EPO group obviously increased over 6 hours,and reached peak 72 hours later,and decreased over 120 hours,and the positive cells in different intervals significantly decreased in ICH group and EPO group compared with those in sham operation group (P < 0.01). The positive cells of TUNEL and Bax in EPO group in different intervals significantly decreased compared with those in ICH group (P < 0.01). The Bcl-2 positive cells in EPO group in different intervals significantly increased compared with those in ICH group (P < 0.01). The Bax protein expression, Bax/Bcl-2 and apoptosis presented positive correlation (P < 0.01). Conclusions Apoptosis may induce some brain injury after ICH,and EPO can decrease the number of apoptotic cells after ICH by up-regulating Bcl-2 and down-regulating Bax.
