CAJ | 학술논문

目的研究富血小板血浆(PRP)在腱骨愈合过程中对肌腱细胞、成骨细胞的增殖情况及胞浆内钙离子浓度的影响.方法用Transwell小室建立共培养模型,用CCK-8试剂盒检测细胞增殖能力,用激光共聚焦显微镜检测经钙离子荧光探针fluo-3/AM染色的胞浆内钙离子浓度的变化.单独培养成骨细胞组、肌腱细胞组,单独培养成骨细胞组、肌腱细胞组并各自加入PRP(加入小室上层),分别以成骨细胞和肌腱细胞为待测细胞建立共培养体系但不加入PRP组,分别以成骨细胞和肌腱细胞为待测细胞建立共培养体系并且加入PRP组. 结果 2种细胞共培养且未加入PRP两组生长速度和荧光强度均为最低,差异有统计学意义(P＜0.05),2种细胞单独培养且未加入PRP的2种细胞生长速度和荧光强度均为居中,差异有统计学意义(P＜0.05),加入PRP的各组生长速度和荧光强度均为最高,且同种细胞间比较差异无统计学意义(P＞0.05).结论 PRP可以去除2种细胞共培养时彼此的抑制效应,并提高细胞的增殖能力至同样高的水平,且胞浆内钙离子浓度也随之升高.
목적 연구부혈소판혈장(PRP)재건골유합과정중대기건세포、성골세포적증식정황급포장내개리자농도적영향.방법 용Transwell소실건립공배양모형,용CCK-8시제합검측세포증식능력,용격광공취초현미경검측경개리자형광탐침fluo-3/AM염색적포장내개리자농도적변화.단독배양성골세포조、기건세포조,단독배양성골세포조、기건세포조병각자가입PRP(가입소실상층),분별이성골세포화기건세포위대측세포건립공배양체계단불가입PRP조,분별이성골세포화기건세포위대측세포건립공배양체계병차가입PRP조. 결과 2충세포공배양차미가입PRP량조생장속도화형광강도균위최저,차이유통계학의의(P＜0.05),2충세포단독배양차미가입PRP적2충세포생장속도화형광강도균위거중,차이유통계학의의(P＜0.05),가입PRP적각조생장속도화형광강도균위최고,차동충세포간비교차이무통계학의의(P＞0.05).결론 PRP가이거제2충세포공배양시피차적억제효응,병제고세포적증식능력지동양고적수평,차포장내개리자농도야수지승고.
Objectlve To explore the effect of platelet-rich plasma (PRP) on cellular proliferation and intracytoplasm calcium ion concentration of osteoblasts and tenocytes during the process of tendon-bone healing. Methods We established a kind of indirect co-culture system using transwell chambers to co-culture osteoblasts and tenocytes.The cellular proliferation was carried out in following manners:single culture of osteoblasts versus single culture of osteoblasts plus PRP and co-culture of osteoblasts versus co-culture of osteoblasts plus PRP; single culture of tenocytes versus single culture of tenocytes plus PRP and co-culture of tenocytes versus co-culture of tenocytes plus PRP.The cellular proliferation rates were measured by CCK-8 test.The intracytoplasm calcium ion concentration of was measured by laser confocal microscopy and fluo-3/AM. Results The proliferation rates and fluorescence intensities in the co-culture groups without PRP were significantly lower than other groups ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the single culture groups without PRP were significantly higher than in the co-culture groups without PRP but significantly lower than in the groups with PRP ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the groups with PRP were significantly higher than other groups ( P ＜ 0.05) but there was no significant difference between groups of the same cells ( P ＞ 0.05).The intracytoplasm calcium ion concentrations were proportional to the proliferation rates. Conclusions During the process of tendon-bone healing,PRP has a potential not only to wipe off the depression effect of osteoblasts and tenocytes co-cultured indirectly but also to enhance the cellular proliferation rate to a higher level.At the same time the calcium ion concentrations will be elevated.