中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2012年
2期
156-159
,共4页
谢谨捷%杨娅%李嵘娟%王艳红%李治安%王征%宋砾%罗向红%苏瑞娟%秦彦文
謝謹捷%楊婭%李嶸娟%王豔紅%李治安%王徵%宋礫%囉嚮紅%囌瑞娟%秦彥文
사근첩%양아%리영연%왕염홍%리치안%왕정%송력%라향홍%소서연%진언문
显微镜检查,声学%动脉粥样硬化%小鼠%载脂蛋白E类
顯微鏡檢查,聲學%動脈粥樣硬化%小鼠%載脂蛋白E類
현미경검사,성학%동맥죽양경화%소서%재지단백E류
Microscopy,acoustic%Atherosclerosis%Mice%Apolipoproteins E
目的 探讨应用超声生物显微镜(UBM)评价高脂饮食下载脂蛋白E(ApoE)基因敲除小鼠动脉粥样硬化的价值.方法 将32只8周龄ApoE基因敲除小鼠分为两组,一组饲以高脂饲料为高脂组,另一组饲以常规饲料作为对照组.饲养8周和16周后,采用Vevo 770超声成像系统观察两组小鼠升主动脉内-中膜厚度(IMT)及动脉粥样硬化斑块面积,并与病理组织学结果和血脂水平进行比较.结果 UBM显示高脂组和对照组小鼠主动脉根部管壁均增厚,出现粥样硬化斑块,但高脂组IMT及斑块面积均较对照组小鼠高,差异具有统计学意义(P＜0.05).UBM所测IMT及斑块面积与病理结果相关性良好(r分别为0.81和0.70)；高脂组小鼠血清甘油三酯(TC)及总胆固醇(TG)水平均较对照组升高(P＜0.05),UBM所测IMT随TC水平升高而增加,两者呈正相关(r=0.528).结论 高脂饮食可促进ApoE基敲除小鼠主动脉粥样硬化斑块形成,超声生物显微镜能作为无创性观测手段来评价活体小鼠的动脉粥样硬化改变.
目的 探討應用超聲生物顯微鏡(UBM)評價高脂飲食下載脂蛋白E(ApoE)基因敲除小鼠動脈粥樣硬化的價值.方法 將32隻8週齡ApoE基因敲除小鼠分為兩組,一組飼以高脂飼料為高脂組,另一組飼以常規飼料作為對照組.飼養8週和16週後,採用Vevo 770超聲成像繫統觀察兩組小鼠升主動脈內-中膜厚度(IMT)及動脈粥樣硬化斑塊麵積,併與病理組織學結果和血脂水平進行比較.結果 UBM顯示高脂組和對照組小鼠主動脈根部管壁均增厚,齣現粥樣硬化斑塊,但高脂組IMT及斑塊麵積均較對照組小鼠高,差異具有統計學意義(P＜0.05).UBM所測IMT及斑塊麵積與病理結果相關性良好(r分彆為0.81和0.70)；高脂組小鼠血清甘油三酯(TC)及總膽固醇(TG)水平均較對照組升高(P＜0.05),UBM所測IMT隨TC水平升高而增加,兩者呈正相關(r=0.528).結論 高脂飲食可促進ApoE基敲除小鼠主動脈粥樣硬化斑塊形成,超聲生物顯微鏡能作為無創性觀測手段來評價活體小鼠的動脈粥樣硬化改變.
목적 탐토응용초성생물현미경(UBM)평개고지음식하재지단백E(ApoE)기인고제소서동맥죽양경화적개치.방법 장32지8주령ApoE기인고제소서분위량조,일조사이고지사료위고지조,령일조사이상규사료작위대조조.사양8주화16주후,채용Vevo 770초성성상계통관찰량조소서승주동맥내-중막후도(IMT)급동맥죽양경화반괴면적,병여병리조직학결과화혈지수평진행비교.결과 UBM현시고지조화대조조소서주동맥근부관벽균증후,출현죽양경화반괴,단고지조IMT급반괴면적균교대조조소서고,차이구유통계학의의(P＜0.05).UBM소측IMT급반괴면적여병리결과상관성량호(r분별위0.81화0.70)；고지조소서혈청감유삼지(TC)급총담고순(TG)수평균교대조조승고(P＜0.05),UBM소측IMT수TC수평승고이증가,량자정정상관(r=0.528).결론 고지음식가촉진ApoE기고제소서주동맥죽양경화반괴형성,초성생물현미경능작위무창성관측수단래평개활체소서적동맥죽양경화개변.
Objective To evaluate the value of ultrasound biomicroscopy (UBM) in assessment of atherosclerosis in apolipoprotein E (ApoE) knockout mice feeding with western diet.Methods Sixteen ApoE knockout mice in 8 weeks age were selected,then divided into two groups.One group was fed with west diet as high-fat group,and another group was fed with normal diet as control group.Intima-media thickness (IMT) and plaque area in the aortic root were assessed by UBM in two groups after 8 weeks and 16 weeks.And the measurements of UBM were compared with results of histopathology and blood-fat.Results Thicken wall and plaque could be find in aortic root in control group and high-fat diet group byUBM.IMT and plaque area in high-fat diet group was significantly higher than those of control group ( P ＜ 0.05).The IMT and plaque area in UBM were good correlation with histopathology ( rwas 0.81 and 0.70 respectively).The triglyceride(TC) and total cholesterol in high-fat diet group was significantly higher than those of control group ( P ＜0.05),and IMT in UBM were increased with the elevated level of TC,there was a positive correlation between IMT and TC( r =0.528).Conclusions Western diet can accelerate the process in formation of atherosclerotic plaque in ApoE knockout mice.UBM can be used to observe this prograss noninvasively in vivo mice.
